---

作 者 ：朱祝军;J.Gerendas;B.Sattelmacher

期 刊 ：植物营养与肥料学报 1997年 3卷 3期 页码：224-228

关键词：缺镍；氮素形态；脲酶；谷氨酰胺合成酶；尿素；水稻；

摘 要 ：采用溶液培养研究了以硝酸铵和尿素作为氮源时，缺镍对水稻植株的生长、脲酶和谷氨酸胺合成酶活性、植株中尿素态N和总氮含量的影响。结果表明，以硝酸铵作为氮源时，缺镍不影响植株的生长;但以尿素作为氮源时植株生长受到明显影响，植株生长停滞，叶片黄化，叶尖坏死干枯。缺镍植株中脲酶活性非常低，地上部仅为供镍植株的0.05％左右;谷氨酰胺合成酶活性除供应尿素氮缺镍植株较低以外，其它处理没有明显差异。缺镍植株中尿素态N含量明显高于供镍植株，特别是供应尿素态氮缺镍植株地上部尿素态N大量累积。供应尿素态氮缺镍植株含氮量明显低于其它处理。

Abstract:The effects of Ni deficiency on growth. activitles of urease and glutamine nynthetuse, the contents of urea and total N in rice plants were studied in solution culture when ammonium nitrate and urea as N sources- The results showed that the growth of rice plant was not affected by Nl deflclency when ammonlum nlttate .as N source. but largely lnhlblted when urea as N source- The actlvltles of urease ln the Nl deflclency plants were very low. The actlvltles of glutamlne Synthetuse were not influenced by the treatments except that its actlvlty ln the plants of urea(-Ni) was decreased. The urea contents were hlgher ln the Nl deflclency plants. eSPeclally urea accumulat6d ln the shoot of urea(-Ni). plants. The total N content was decreased ln the plants of urea(-Nll compared wlth other treatInents.

---