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Photosynthetic Characteristics and Chlorophyll Fluorescence Parameters of Celtis sinensis and Ulmus pumila L. Seedling under Salt Stress

盐胁迫对朴树和速生白榆幼苗光合特性及叶绿素荧光参数的影响



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,2016,36(5):1004-1011
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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犘犺狅狋狅狊狔狀狋犺犲狋犻犮犆犺犪狉犪犮狋犲狉犻狊狋犻犮狊犪狀犱犆犺犾狅狉狅狆犺狔犾犉犾狌狅狉犲狊犮犲狀犮犲犘犪狉犪犿犲狋犲狉狊狅犳
犆犲犾狋犻狊狊犻狀犲狀狊犻狊犪狀犱犝犾犿狌狊狆狌犿犻犾犪犔.犛犲犲犱犾犻狀犵狌狀犱犲狉犛犪犾狋犛狋狉犲狊狊
ZHOUDandan1,LIUDexi2,LICunhua1,CHENMiaomiao1,LIUGuimin2,
YANGQingshan2,LIYongtao2
(1ColegeofForestryShandongAgriculturalUniversity,Tai’an,Shandong271018,China;2ShandongAcademyofForestry
Sciences,Jinan250014,China)
犃犫狊狋狉犪犮狋:Theannual犆犲犾狋犻狊狊犻狀犲狀狊犻狊and犝犾犿狌狊狆狌犿犻犾犪 L.seedlingsastestmaterialtomeasurethe
growth,chlorophylcontent,photosyntheticcharacteristicsandchlorophylfluorescenceparametersof犆.
狊犻狀犲狀狊犻狊and犝.狆狌犿犻犾犪Thedesiredsaltconcentrationismadeup34.6‰oftheundergroundnaturalsaline
water,thesoilreached2‰,2.5‰,3.5‰and5‰salinityseparately.Thegrowth(heightandgrounddi
ameter),varietiesofchlorophylcontent,photosyntheticcharacteristicsandchlorophylfluorescencepa
rametersof犆.狊犻狀犲狀狊犻狊and犝.狆狌犿犻犾犪weremeasuredafter45daystostudytheirsalttolerance.There
sultsshowedthat:(1)thegrowthof犝.狆狌犿犻犾犪wasalwayshigherthanthatof犆.狊犻狀犲狀狊犻狊inalsalt
stresstest.The犆.狊犻狀犲狀狊犻狊chlorophylcontentincreasedfirst,andthendecreased,but,chlorophylcon
tentof犝.狆狌犿犻犾犪lessthancontrolgroupanddeclinedineverysalttreatment.(2)Underdifferentsalt
stress,initialfluorescence(犉o),maximalfluorescence(犉m)andphotochemicalefficiencyofPSⅡ (犉v/
犉m)of犆.狊犻狀犲狀狊犻狊and犝.狆狌犿犻犾犪aredropped.Comparethesetwospecies,thesefluorescenceparameters
(犉狅,犉犿,犉狏/犉犿)of犝.狆狌犿犻犾犪slightlydecrease,but,thenonphotochemicalquenchingcoefficient(NPQ)
of犝.狆狌犿犻犾犪increasedsignificantlythanthatof犆.狊犻狀犲狀狊犻狊whensoilsaltcontentmorethan2.5‰.The
chlorophylcontentof犆.狊犻狀犲狀狊犻狊isalwayshigherthanthatof犝.狆狌犿犻犾犪undereverysaltstress,butthe
chlorophylfluorescenceislowerunderhighersaltstresscondition(>2.5‰);Undertheconditionofnon
stressandlowersaltstress,犆.狊犻狀犲狀狊犻狊leavespossessbetterphotochemicalperformancethanthatof犝.
狆狌犿犻犾犪leaves.Inconclusion,whensoilsaltcontentwashigher(>2.5‰).thechangesofthephotosyn
theticandfluorescenceparametersof犝.狆狌犿犻犾犪morestablethanthatof犆.狊犻狀犲狀狊犻狊.Consequently,under
saltstressenvironment,犝.狆狌犿犻犾犪canbetteradapttosaltstressenvironmentthan犆.狊犻狀犲狀狊犻狊.
犓犲狔狑狅狉犱狊:犆犲犾狋犻狊狊犻狀犲狀狊犻狊;犝犾犿狌狊狆狌犿犻犾犪L.;saltstress;chlorophylcontent;photosyntheticcharacteris
tics;chlorophylfluorescence
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Tabe1 Tableoffivesaltgradientspreparationsodium
wV>?
Number
ƒ±nŒ
4ˆ
Requiredsoil
saltcontent/‰
/Œ
ˆNot
wateringsoil
moisture/%
ˆ19%
ƒ¿ˆ
19% moisturecontent
needwater/kg
‚‰4
„…
Needsaltwater
concentration/‰
‚¿6|4

Requiredunderground
saltwater/kg
‰•–†Œ
4ˆ
Testedsoilsalt
content/‰
1 0 13.5 3400 0 0 0
2 2.0 13.5 3400 3.5 339.507 2.063
3 2.5 13.5 3400 4.5 437.92 2.603
4 3.5 13.5 3400 6.0 824.032 3.565
5 5.0 13.5 3400 9.0 880.75 5.014
  ²:wVŒöv70129.75kg,}~6|4ˆv34.6‰
Note:Soilweightoftesttankis70129.75kg.Theundergroundsaltwaterconcentrationis34.6‰
“”’[nŒ4ˆ+@r6sjx
(3.5‰~
4.5‰),ݵ’[“3µö³,6&v1ö³Œs。
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v34.6‰n6|}~4€‚ƒ4„…。v
±ÔênŒ4ˆÝµwVƒ‰4ˆv
0‰、3.5‰、4.5‰、6.0‰、9.0‰}~6|4。´
‡4íî¡4{®…µ¶Š·4ˆ

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15min•,¶Ð,y80%ÁÂÑÒÓԖ†ÕÌ
25mL,Ãց T{Ž×663nm\645nm、ט
’–†Øp
,¸
ÙÚÛÜôš›œ„…\ˆ

š›œ„…
(mg·L-1)=(12.72A663-2.59
A645)+(22.88A645-4.67A663)
š›œˆ
(mg·g-1)=š›œ„…(mg·
L-1)ׁ TÝÞî(L)/ÃÖÄö(g)
1.3.2 FsABtuvwJK xyLi6000XT
ßà۝žOyÛ4–†#&šzn᝞qÎ
Pn、âãW…Gs、ä*CO2 „…Ci\åæqÎTr。
–†½v1000μmol·m
-2·s-1,ç*výè
10∶00~11∶30,#&םÙ|˜{éê•×éê
hø–†

–†ç+ J|}ýë3{ìníîï
ðñ®š

Ýp*ݒ[10µö³。
1.3.3 FGHIAJK yLi6000XTßà۝
žOyÛ4n¢ò–Û4–†ìã¸ó3µ3ô
šznš›œ¢Ÿ 

 ÀÏp
)。
õšz=éê
30min,–†ÉÊ¢(犉狅)、Ëd¢(犉犿)、PSⅡË
d¦%ÍÎ
(犉狏/犉犿),ù•høéênŸ –
†
,¯
èö¢
(犉狊)、éê|Ëd¢(犉犿′)、
éê|ÉÊ¢
(犉狅′),–µ¶Ø¦%ÙÚÛ 
(犖犘犙)¡¦%ÙÚÛ (狇犘)。
1.4 Kxcd
ySPSS18.0\Excel2007hø4µ{DŸ

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2 °±Ó{D
2.1 yz4{hcd789:;<=>?@qLMN
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a×3.5‰\5.0‰4“”|²ÞÀ,opt
unu7

6™Ô©G˜Î{ŽÃÄ35.8%~
53.6%\30.2%~44.4%,a×2.0‰~5.0‰4“
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6001 ! " # $ % &                   36ü
e2 yz456|89:;<=>?@L}7Tabe2 Therelativegrowthrateof犆.狊犻狀犲狀狊犻狊and犝.狆狌犿犻犾犪seedlingsunderdifferentsaltstress
p*
Species
Ô©G˜Î
Relativegrowth/%
Œ4ˆSoilsaltcontent/‰
0(CK) 2.0 2.5 3.5 5.0
qGrs
犝.狆狌犿犻犾犪L.spp.
u7 Height 65.79±4.69a 60.00±3.59a 52.25±2.16a 42.23±3.74b 39.80±3.13c
6™Grounddiameter 70.93±3.61a 68.62±1.96a 66.90±2.87a 51.58±2.01b 44.10±3.01c
op
犆.狊犻狀犲狀狊犻狊
u7 Height 51.94±1.94a 42.08±1.23b 35.07±0.56c 33.33±0.61c 24.10±1.12d
6™Grounddiameter 47.80±3.16a 42.40±2.08b 39.83±2.89b 33.35±3.01c 26.60±2.96d
  ²:и¨ø ‡‚L§¨Œýþÿ!Ч¨„…4’[*n÷ø0.05²ÞÀ
  Note:Thedifferentlowercaselettersindicatethesignificantdifferencesamongdifferentsaltstressesat0.05level
§¨Œýþÿг’[*÷ø0.05²ÞÀ
Ÿ2 4“”©op\qGrstuš›œˆnª«
Thedifferentlowercaselettersreflectsignificantdifferences
amongtreatmentsat0.05level
Fig.2 Effectsofsaltstressonchlorophylcontentin
seedlingsof犆.狊犻狀犲狀狊犻狊and犝.狆狌犿犻犾犪
2.2 yz4{hcd789:;<=>FGHr
qLMN
  Ÿ2²³,»¼4“”’[„…n¾¿,opt
ušznš›œˆÀÑÁ¾¿•ÃÄn¥¦Å
Æ

–×2.0‰\2.5‰n4“”’[|{ŽÕCK
²Þ¾¿þ18.04%\16.48%(犘<0.05),×
3.5‰\5.0‰’[|{ŽÕCK|Ãþ4.0%8.67%;¨ ç,qGrsnš›œˆÇÐÑÒÁÃ
•Ân¥¦ÅÆ

º×‘½…4“”’[|ϲÞ
ÄÈCK,Ãûv18.23%~55.38%,–×5.0‰’
[|š›œˆ
(0.729mg·g-1)±ËÄÀ。


‘’[*qGrsnš›œˆÏRײÞ
¤÷ø

aÔ¨’[|#p**š›œˆ$R×
²Þ¤÷ø

lý°±%ÖqGrsnš›œˆ
ב„…4“”|Ïì±²Þ(

©4“”Ü&


optušznš›œˆ×Ą…4“”
|>²Þ7

(×7„…|ì±²Þ(

2.3 yz4{hcd789:;<=>PAB
JKLMN
2.3.1 €AB;~ »¼4“”„…n¾¿,qG
rs\optušz᝞qÎ
(犘狀)ÏÐÑÒÁ
)*ý•|Ãn¥¦ÅÆ

Ð3)。—¸,qGr
stušz犘狀 ×2.0‰4„…’[|±Ëdp,
qçÕCK²Þ¾¿20.88%;+4“”„…dÈ
2.0‰ç,qGrs犘狀 ÏÄÈCK,–×5.0‰„…
’[|±ËÄp

qç²ÞÄÈCK,31.4%;
optušz犘狀 ×2.5‰„…’[珱Ëdp,
qçÕ CK ¾¿1.20%(犘>0.05),×3.5‰5.0‰„…’[|ÕCK{Ž²ÞÃÄ35.03%46.82%。×Ô¨’[„…|,optušz犘狀 7
ÈqGrs

º(×CK\2.5‰’[|R×-²
Þ÷ø
(犘<0.01);optu犘狀 ×¹74„…“”
|Ü.ì±(|Ã

a|Ãû…dÈqGrs

2.3.2 t‚h:ƒ„…†‡ˆ{h Ð3²³,
âãW…
(犌狊)Ó#$šz/®¯\CO2 „…æ
0

qGrs\opšzn犌狊 ¥¦ÅÆÔ1,Ï»
¼4“”½…n¾¿Á¾¿•|Ã

qGrs犌狊
×2.0‰„…“”珱23,Õ CK ²Þ¾¿
50%,•)*|Ã,–ÓCK÷ø§²Þ;op犌狊×
2.5‰4„…“”’[|±Ëdp,ºÓCK÷ø
§²Þ

×3.5‰\5.0‰4“”|{ŽÕCK²
Þ|Ã49.03%\67.74%。¨ç,op\qGrs
šz犆犻 ב„…4“”’[|ϲÞ7ÈÔê
CK,45n¾û{Žv 17.31% ~20.51% 8.65%~14.21%,qGrs犆犻 ב4“”’[*
÷ø²Þ

op犆犻 ב4“”’[*™²Þ÷
ø

|3.5‰’[ê,—6’[|qGrs犆犻 ϲ
ތÈÔêop



×>2.5‰’[|op×
犌狊|Ã78|,犆犻 9ÒÑÂ7Ñ:。%Ö,×¹7
4„…n4“”|

Øâãëœ÷op᝞qÎ
ÃÄnHWëœ

2.3.3 ‰Š;~:‹VŒŽ~ JÐ3Hù,q
Grsšz×2.0‰„…’[|犜狉±Ëd,–²
70015‡         @AA,%:4“”©op\qGrstuž£¤¡š›œ¢Ÿ nª«
e3 456789:;<=>?@犘狀、犌狊、犆犻、犜狉、犠犝犈 LMN
Table3 Effectsofsaltstresson犘狀,犌狊,犆犻,犜狉and犠犝犈of犆.狊犻狀犲狀狊犻狊and犝.狆狌犿犻犾犪seedlings
p*
Tree
species
Œ4ˆ
Soilsalt
content/‰
犘狀
/(μmol·m-2·s-1)
犌狊
/(μmol·m-2·s-1)
犆犻
/(μmol·mol-1)
犜狉
/(mmol·m-2·s-1)
犠犝犈
/(μmol·mmol-1)
qGrs
犝犾犿狌狊
狆狌犿犻犾犪
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3.5 13.50±3.512c 0.158±0.767c 264.13±12.247a 5.71±0.77a 2.45±0.827b
5.0 11.06±3.236c 0.10±0.078c 259.83±8.030a 3.30±0.766c 3.57±1.939a
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