全 文 :书!"#$%&
,2016,36(8):1507-1514
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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h)½¾Ô,ÕÒ6犞狏犆γ犞犘犈 ,ÈË_Ì4hÆÏÅÖ×,ØÌÙÚ。γ犞犘犈,È
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犆犾狅狀犵犻狀犵犪狀犱犈狓狆狉犲狊狊犻狅狀犃狀犪犾狔狊犻狊狅犳γ犞犘犈犌犲狀犲犚犲犾犪狋犲犱
狋狅犘狅狑犱犲狉狔犕犻犾犱犲狑犚犲狊犻狊狋犪狀犮犲犻狀犌狉犪狆犲狏犻狀犲
WANGYanqing,ZHANGXiaoying,SONGJianing,WANGYuejin,ZHANGChaohong
(ColegeofHorticulture,NorthwestA&FUniversity,StateKeyLaboratoryofCropStressBiologyinAridAreas,KeyLabora
toryBiologyandGeneticImprovementofHorticultureCrops,MinistryofAgriculture,Yangling,Shaanxi712100,China)
犃犫狊狋狉犪犮狋:Vacuolarprocessingenzyme(γ犞犘犈)wasclonedfromChinesewild犞犻狋犻狊狆狊犲狌犱狅狉犲狋犻犮狌犾犪狋犪acces
sion.‘Baihe351’and犞犻狋犻狊狏犻狀犻犳犲狉犪cv.‘Carinena’byRTPCR.Theywerenamed犞狆γ犞犘犈 and
犞狏犆γ犞犘犈.Thefullengthof犞狆γ犞犘犈and犞狏犆γ犞犘犈cDNAare1624bp,andtheopenreadingframeare
1482bp,encoding493aminoacid.ThealignmentoftheaminoacidsequencerevealedthatSer395,one
keyaminoacidinsubstratepocket,wasreplacedbyAlain犞犻狋犻狊狆狊犲狌犱狅狉犲狋犻犮狌犾犪狋犪accession.‘Baihe351’,
犞犻狋犻狊狏犻狀犻犳犲狉犪cv.‘Carinena’,犞犻狋犻狊狏犻狀犻犳犲狉犪cv.‘Thompsonseedless’,and犞犻狋犻狊狏犻狀犻犳犲狉犪cv.‘Pinot
Noir’.Thedifferentexpressionprofileof犞狆γ犞犘犈 geneand犞狏犆γ犞犘犈 geneindifferentperiodsafter
powderymildewinducedwereanalyzedbyRealtimefluorescentquantitativePCR.Afterinductiontheex
pressionof犞狆γ犞犘犈geneincreaseslightlyin4h,48h,168handthe犞狏犆γ犞犘犈geneshowspredominant
expressionin4hafterthatitdecreasesslowly.Thestudyshowsthatγ犞犘犈isrelatedtoresistanttopow
derymildew.Thestudyprovidesreferencevaluetothemolecularmechanismofγ犞犘犈geneindiseasere
sistance.
犓犲狔狑狅狉犱狊:grape,γ犞犘犈,geneclone,expressionanalysis,powderymildew
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▲.犞狆γ犞犘犈genein犞犻狋犻狊狆狊犲狌犱狅狉犲狋犻犮狌犾犪狋犪accession.‘Baihe351’;●.犞狏犆γ犞犘犈geneinV犻狋犻狊狏犻狀犻犳犲狉犪cv.‘Carinena’;Thenumbers
onthebranchesrepresentthepercentagesoftimesthatthespeciesaregroupedtogetherinbootstrapanalysisfor1000replicates
Fig.2 Phylogenetictreesof犞狆γ犞犘犈geneand犞狏犆γ犞犘犈geneofgrape
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accession.Baihe351;VvC.犞犻狋犻狊狏犻狀犻犳犲狉犪cv.Carinena;▲.Catalyticdyad;◆.Threekeyaminoacids;.Themutationsite
Fig.3 Alignmentoftheaminoacidsequencesof犞狆γ犞犘犈,犞狏犆γ犞犘犈geneingrapewithother犞犘犈genes
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