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Analysis of Genetic Diversity of Germplasm Resources of Lycium barbarum L.Revealed by SRAP in Xinjiang of China

新疆枸杞种质资源遗传多样性的SRAP分析



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IJBK:Q346+.5 !LMNO:A 犃狀犪犾狔狊犻狊狅犳犌犲狀犲狋犻犮犇犻狏犲狉狊犻狋狔狅犳犌犲狉犿狆犾犪狊犿犚犲狊狅狌狉犮犲狊狅犳犔狔犮犻狌犿 犫犪狉犫犪狉狌犿犔.犚犲狏犲犪犾犲犱犫狔犛犚犃犘犻狀犡犻狀犼犻犪狀犵狅犳犆犺犻狀犪 ZHAMeiqin1,ZHAOYulin2,LIJiang1,ZHUYu1,LUOShuping1,HUYue1 (1.ColegeofForestryScienceandHorticulture,XinjiangAgriculturalUniversity,Urumqi830052,China;2.ManagementCen terofJingheCountyWolfberry,Jinghe,Xinjiang833300,China) 犃犫狊狋狉犪犮狋:SRAPmarkerswereusedtostudythegeneticdiversityandphylogeneticrelationshipforpro vidindatheoreticalbasisforidentificationandcrossbreedingofgeneticresourcesof犔狔犮犻狌犿犫犪狉犫犪狉狌犿L.in XinjiangofChina.Theresultsare:(1)atotalof310lociwereproducedby12pairsofSRAPprimers,and polymorphiclociwere264whichaccountedfor84.61%inthetotalamplifiedfragments.Thepolymor phisminformationcontent(犘犐犆)valuesofthesemarkersvariedfrom0.76to0.93withanaverageof0. 83.Meanvaluesofobservednumberofaleles(犖犪),effectivenumberofaleles(犖犲),Nei’sgenediversity (犎)andShannon’sinformationindex(犐)were1.8461,1.3869,0.2280and0.3520.(2)Rangeofgenet icsimilarity(犌犛)was0.5903to0.9032among30wolfberrysamples.Clusteranalysisshowedthatsam plescouldbedividedinto2groupsand5subgroupsaccordinglyatthe犌犛of0.70and0.76.Theprincipal coordinateanalysisandclusteringresultsarebasicalyconsistent.Researchshowsthegeneticdiversityof germplasmresourcesof犔.犫犪狉犫犪狉狌犿L.inXinjiangwasrelativelyabundant.Thegeneticdifferencebe tweenwildspeciesandcultivars(lines)wasrelativelylarge,showadistantrelationship,andthegenetic differenceamongcultivars(lines)wasrelativelysmal,showacloserelationship. 犓犲狔狑狅狉犱狊:犔狔犮犻狌犿犫犪狉犫犪狉狌犿L.;sequencerelatedamplifiedpolymorphism(SRAP);clusteranalysis;prin cipalcoordinatesanalysis;geneticdiversity;   jk(犔狔犮犻狌犿犫犪狉犫犪狉狌犿 L.)ÔÕ/(Solanace ae)jkÖ(犔狔犮犻狌犿L.)’Â[y×IØÙÚN[1]。 jkSÂ[ÛÜÝ]#

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[J].w¢^*/%,2013,41(4):14591461.
CHENG,LIUJ,MAZG,犲狋犪犾.Analysisofwolfberry(犔狔犮犻狌犿
L.)inGansubyRAPD[J].犑狅狌狉狀犪犾狅犳犃狀犺狌犻犃犵狉犻犮狌犾狋狌狉犪犾
犛犮犻犲狀犮犲,2013,41(4):14591461.
[2] £ ¤,U ¥,¦dð.´§jkwxyz-’RAPDr±
[J].#0H ¨©ðª«¬­ ®¯jkÖ[>]{tQ0H
€
[J].nnۄ|,2008,33(18):20202027.
DONGJZ,YANGJJ,WANGY.Resourcesof犔狔犮犻狌犿species
andrelatedresearchprogress[J].犆犺犻狀犪犑狅狌狉狀犪犾狅犳犆犺犻狀犲狊犲
犕犪狋犲狉犻犪犕犲犱犻犮犪,2008,33(18):20202027.
[4] °|œ,±²),³´µ.jk"Ý[\’nrDNAITSÒÓr
±0H
[M]."è:n)*‘¶·,2012:191.
686 ! " # >]¿@{™™†
ÀÁ
[J]."3ab,2013,(3):169171.
WANGZJ,JINKY,GULS.Resourceinvestigationandcon
servationmeasuresof犔狔犮犻狌犿 L.inXinjiang[J].犖狅狉狋犺犲狉狀
犎狅狉狋犻犮狌犾狋狌狉犲,2013,(3):169171.
[6] ºÃ,UÐÄ,­f,¨ .jkwxyz-’ISSRr±[J].
ù"^%&
,2014,29(1):8992.
BAOHC,LIXL,WANGJP,犲狋犪犾.ISSRanalysisofgenetic
diversityofChinesewolfberrygermplasm[J].犃犮狋犪犃犵狉犻犮狌犾狋狌
狉犪犲犅狅狉犲犪犾犻犛犻狀犻犮犪,2014,29(1):8992.
[7] Å Æ,°|œ,ÇÈ´,¨ .jkwxÉ£0H€[J]."3
ab
,2012,(24):187191.
QUL,SHIZG,JIAOEN,犲狋犪犾.Advancesontransformation
ofwolfberry[J].犖狅狉狋犺犲狉狀犎狅狉狋犻犮狌犾狋狌狉犲,2012,(24):187191.
[8] UÊË,ÌÍÎ,ÏÐ,¨ .jk[\wxyz-’ AFLPr
±
[J].nÑÛ,2011,42(4):770773.
LIYL,FANYF,DAIGL,犲狋犪犾.Analysisofgeneticdiversi
tyforwolfberrygermplasmsbyAFLPtechnology[J].犆犺犻狀犲狊犲
犜狉犪犱犻狋犻狅狀犪犾犪狀犱犎犲狉犫犪犾犇狉狌犵狊,2011,42(4):770773.
[9] LIG,QUIROSCF.Sequencerelatedamplifiedpolymorphism
(SRAP),anewmarkersystembasedonasimplePCRreac
tion:itsapplicationtomappingandgenetaggingin犅狉犪狊狊犻犮犪
[J].犜犺犲狅狉犲狋犻犮犪犾犪狀犱犃狆狆犾犻犲犱犌犲狀犲狋犻犮狊,2001,103:455461.
[10] ›%¬,ÒÓù,¨ .nÚNmn[\wxyz-’SRAP
ƒSSRr±[J].!"#×Ø,¨ ."Œìoprswxcd’e
f{r±
[J].!"#%&,2015,35(5):915921.
LIUXY,HUOXW,YANGM,犲狋犪犾.Geneticdiversityanal
ysisandprimarycorecolectionconstructioninyam(犇犻
狅狊犮狅狉犲犪狅狆狆狅狊犻狋犪Thunb.)byISSRmarker[J].犃犮狋犪犅狅狋.犅狅
狉犲犪犾.0犮犮犻犱犲狀狋.犛犻狀.,2015,35(5):09150921.
[13] Íæß,ސß,àFá,¨ .nïrßï^(¾[·’w
xGÌ-r±ƒ DNA­âcdef[J].#wx>]wx>]%&,2013,14(4):627633.
YUANHJ,ANW,LILH,犲狋犪犾.Theinvestigationandclus
teranalysisofmainmorphologicalcharactersforgermplasm
ofChinese wolfberry [J].犑狅狌犿犪犾狅犳 犘犾犪狀狋犌犲狀犲狋犻犮犚犲
狊狅狌狉犮犲狊,2013,14(4):627633.
[15] Yæç,ŸÖè,é†ê,¨ .jk’vwxg#r±[J].n
nۄ|
,1997,22(9):532533.
JIANYX,MA YZ,PENG Y H,犲狋犪犾.Analysisofchro
mosmekaryotypeof犔狔犮犻狌犿犮犺犻狀犲狀狊犲 Mil.[J].犆犺犻狀犪犑狅狌狉
狀犪犾狅犳犆犺犻狀犲狊犲犕犪狋犲狉犻犪犕犲犱犻犮犪,1997,22(9):532533.
[16] Ÿæ,ëßØ,ªòì.jky[ÝyzL’wxyz-

[J].w¢^*/%,2010,38(8):40424043.
MAYP,ZHAOHY,YANGSJ.Analysisofthegeneticdiversi
tyofChinesewolfberryatthelevelsofvariousisozyme[J].犑狅狌狉
狀犪犾狅犳犃狀犺狌犻犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲,2010,38(8):40424043.
[17] ¦í«,› î,ï ð,¨ ."Ýñòõpn´§jkÙI
mGóƒRAPDr±[J].nôõ,2005,25(3):391396
ZHANGM X,CHENT,XIAO W,犲狋犪犾.Physiologicalfea
turesandRAPDanalysisof犔狔犮犻狌犿犫犪狉犫犪狉狌犿leavesindif
ferentsalinehabitats[J].犑狅狌狉狀犪犾狅犳犇犲狊犲狉狋犚犲狊犲犪狉犮犺,2005,
25(3):391396.
[18] ö÷÷,­ ø.ISSRPCRrstuƒi¯´k1¦`ù-
"†jk
[J].w¢^*/%,2011,39(14):83098310.
SIBB,WANGZ.IdentificationofNo.1andmalesterilityof
犔狔犮犻狌犿犫犪狉犫犪狉狌犿 L.byISSRPCR molecularmarkertech
nique[J].犑狅狌狉狀犪犾狅犳犃狀犺狌犻犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲,2011,39
(14):83098310.
[19] úûü,ý,¨ .pqSSROPv17½jkµ¶€.w xyz-r±{t“­âcdef [J]."3ab,2015, (12):9195. SHAOQS,GAOL,犲狋犪犾.Analysisofgeneticdiversityand constructionoffingerprintof犔狔犮犻狌犿犫犪狉犪狉狌犿 L.usingSSR technology[J].犖狅狉狋犺犲狉狀犎狅狉狋犻犮狌犾狋狌狉犲,2015,(12):9195. [20] þˬ,³´µ,y z,¨ .jk¾[SRAPr±[J].´§^ )/O ,2014,55(12):2022. WULJ,MENHQ,YINY,犲狋犪犾.Analysisof犔狔犮犻狌犿犫犪狉犫犪狉狌犿 varietiesbyusingSRAP[J].犖犻狀犵狓犻犪犑狅狌狉狀犪犾狅犳犃犵狉犻犮狌犾狋狌狉犲犪狀犱 犉狅狉犲狊狋狉狔犛犮犻犲狀犮犲犪狀犱犜犲犮犺狀狅犾狅犵狔,2014,55(12):2022. [21] ÿ !,"#!,f3,¨ .%pDDNA"݇Á3ƒ’0
H
[J].34^*_%%&,2006,29(2):4346.
LAP,LUOSP,ZHANGJX,犲狋犪犾.Studiesondifferent
methodsofextractingsugarbeettotalDNA[J].犑狅狌狉狀犪犾狅犳
犡犻狀犼犻犪狀犵犃犵狉犻犮狌犾狋狌狉犪犾犝狀犻狏犲狉狊犻狋狔,2006,29(2):4346.
[22] ª&Ø,,™,¾(),¨ .*NrJɾ[SCoT­âcde
f{wx¢Ìr±
[J].!"#%
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