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作 者 ：杨明琰 1,田 稼 2,马 瑜 2,孙 超 2,黄继红 2

期 刊 ：西北植物学报 2012年 1期 页码：193~198

关键词：杜仲；内生真菌；分离；分类鉴定；抗菌活性；

Keywords:Eucommia ulmoides Oliv., endophyte fungi, isolation, identification, antimicrobial activity,

摘 要 ：采用组织分离法从秦岭药用植物杜仲茎部共分离到内生真菌38株，经形态学鉴定分属于9属，其中青霉属为杜仲内生真菌中的优势菌群，拟青霉属、交链孢属和无孢群属为常见属。对分离得到的杜仲内生真菌发酵液进行了抑菌实验，其中61%的菌株对测试细菌具有抗菌活性，表明杜仲内生真菌的抗菌活性十分普遍；34%的菌株对烟草赤星病菌、苹果炭疽病菌及辣椒炭疽病菌等多种植物病原菌具有较强的抑制活性，表明杜仲内生真菌在植物病害的生物防治领域具有进一步开发的价值。

Abstract:38 strains of endophyte fungi belong to 9 genera were isolated from the stem of medicinal plant Eucommia ulmoides Oliv.collected in Qinling Mountain by the methods of tissue separation.Penicillium was the dominant group and Paecilomyces,Alternaria,mycelia sterilia were the common groups.The result of antimicrobial activity assay showed 61% endophyte fungi presented antibacterial activity,34% endophyte fungi presented antifungals activity towards Algernaria alternata,Colletotrichum gloeosporioides and C.capsici.It indicated antimicrobial activity was common in endophyte fungi isolated from E.ulmoides Oliv.and worthing for further studying in biological control of plant diseases.

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