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作 者 ：万东莉，丛靖宇，李瑞丽，邹 博，王瑞刚，李国婧*

期 刊 ：西北植物学报 2012年 6期 页码：1099~1105

关键词：拟南芥；钙调素结合蛋白；CBP60g；基因表达；胁迫；

Keywords:Arabidopsis thaliana, calmodulin binding protein, CBP60g, gene expression, stress,

摘 要 ：采用实时荧光定量RT PCR和Northern blotting技术检测了野生型拟南芥中CBP60g基因对丁香假单胞菌和非生物胁迫的响应，并对丁香假单胞菌接种后，野生型拟南芥、cbp60g 1突变体和CBP60g过表达转基因植物中抗逆相关基因的表达变化进行检测。结果显示：（1）在野生型拟南芥中CBP60g基因的表达能被丁香假单胞菌、高盐、冷和机械损伤所诱导。（2）经丁香假单胞菌诱导后病程相关基因PR5和AIG1的表达在过表达转基因植物中明显高于野生型。（3）受干旱和ABA诱导的AtMYB2基因的表达在过表达转基因植物中也高于野生型。研究表明，CBP60g同时参与了拟南芥对生物和非生物胁迫响应。

Abstract:In this study,real time PCR or Northern blotting was employed to analyze the expression patterns of the CBP60g gene in wild type Arabidopsis thaliana after Pseudomonas syringae and abiotic stress treatment.In addition,the expression of pathogen induced genes in wild type,cbp60g 1 mutant and CBP60g overexpression plants after P.syringae inoculation were compared.The results showed:(1)Transcript of CBP60g was induced by P.syringae,high salt,cold and wounding treatment in wild type plants.(2)The expression of pathogen induced genes PR5 and AIG1 in the CBP60g overexpression plants was enhanced after P.syringae inoculation and was reduced in the mutant.(3)AtMYB2,an abiotic stress induced gene reported previously,was also induced by Pst DC3000,and reached to a higher level in overexpression plants than in wild type.Taken together,these results indicate that CBP60g involved in A.thaliana multiple stresses response.

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