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作 者 ：王雅琴1,石 淼1,张新宇2,刘永昌2,薛 飞2,孙 杰2,李艳军2*

期 刊 ：西北植物学报 2013年 5期 页码：878~884

关键词：棉花；MYB转录因子；非生物胁迫；

Keywords:cotton, MYB transcription factors, abiotic stress,

摘 要 ：该研究利用序列拼接并结合RT-PCR技术,从棉花叶片中克隆了1个MYB基因的cDNA序列,命名为GhMYB113。序列分析表明,该基因开放阅读框为738 bp,编码246个氨基酸,含有2个MYB结构域,属R2R3-MYB类型转录因子。该基因的基因组序列长1 927 bp,由3个外显子和2个内含子构成。氨基酸序列比对发现该蛋白与其他物种的MYB蛋白有较高的一致性。系统进化分析显示,棉花GhMYB113与现代杂交月季亲缘关系最近。qPCR分析发现，该基因在棉花根中优势表达,在干旱、高盐及低温胁迫后表达量均发生变化,推测GhMYB113可能在植物响应干旱、高盐及低温等非生物胁迫过程中起作用。

Abstract:In this study,the cDNA of a new MYB gene,designated as GhMYB113,was isolated from cotton leaf by in silico cloning and RT-PCR.The length of its ORF was 738 bp and encoded a protein with 246 amino acids.The genomic sequence of GhMYB113 was 1 927 bp in length and was composed of 3 exons and 2 introns.Predicted protein belonged to R2R3-MYB family and contained two MYB domains.Alignment of sequences showed that the deduced GhMYB113 protein was highly homologous to other MYB proteins from different species.Phylogenetic analysis indicated that GhMYB113 was closest to MYB of hybrid rose.The quantitative real-time PCR (qPCR） demonstrated that GhMYB113 highly expressed in root.After drought,salt and low-temperature stress treatment,qPCR is perfomed to detecting the expression pattern of GhMYB113 in cotton seedlings.It turned out that GhMYB113 responded to all the three kinds of abiotic stresses.It was speculated that GhMYB113 plays a important role in response to abiotic stresses including drought,salinity and low-temperature .

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