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作 者 ：李荣冲，沈亮余，梁晶龙，赵敬会，王瑞雪，邹 燕，张 涛 *

期 刊 ：西北植物学报 2012年 2期 页码：312~317

关键词：紫苏；种子萌发；茉莉酸甲酯；高温高湿；抗氧化酶；

Keywords:Perilla frutescens, seed germination, MeJA, high temperature and air humidity, antioxidant enzyme,

摘 要 ：以紫苏品系GS011种子为材料，在温度为50℃、空气相对湿度为95%、时间为12 h的胁迫条件下，研究不同浓度（0.05、0.10、0.15、0.20和0.25 mmol·L^-1）茉莉酸甲酯（MeJA）对紫苏种子萌发、幼苗生长、叶片氧化损伤和抗氧化酶活性的影响。结果表明：0.15 mmol·L^-1 MeJA处理能显著缓解高温高湿对紫苏种子生长发育造成的胁迫伤害，使紫苏种子发芽率、发芽势、发芽指数、活力指数均达到最大值，分别为40.84%、31.83%、8.83和1.35；同时使其叶片MDA含量最低（0.15 μmol·g^-1），SOD、CAT、LOX、POD和APX活性均达到最大值，分别为0.27 U·mg^-1及4.7、5.7、4.9和8.9 U·g^-1·min^-1。研究发现，MeJA在一定程度上能提高SOD等保护酶的活性，缓解高温高湿造成的氧化损伤，有效促进高温高湿胁迫下紫苏种子的萌发和幼苗生长。

Abstract:The effects of exogenous MeJA at five concentrations of 0.05,0.10,0.15,0.20 and 0.25 mmol·L^-1 on seed germination,seedling growth,the oxidative damage and the protective enzyme activities of the leaves of Perilla frutescens were measured under high air humidity (95%) for 12 hours at 50℃.The results indicated that the damages to the P.frutescens caused by the high temperature and air humidity were most significantly relieved by the 0.15 mmol·L^-1 MeJA.The seed germination rate,germination potentiality,germination index and vigor index increased to the maximum with 40.84%,31.83%,8.83 and 1.35,respectively.MDA content decreased to the minimum with 0.15 μmol·g^-1.The protective enzymes of SOD,CAT,LOX,POD and APX increased to the maximum with 0.27 U·mg^-1,4.7,5.7,4.9 and 8.9 U·g^-1·min^-1,respectively.Based on the data reported,we conclude that MeJA can relieve oxidative damage under high temperature and high air humidity stress,enhance the activity of protective enzymes and stimulate P.frutescens seed germination and seedling growth.

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