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作 者 ：高 义1，李虎强1，张志军2，李晓君2*

期 刊 ：西北植物学报 2013年 7期 页码：1473~1477

关键词：紫苏；内生真菌；化学成分；生物活性；

Keywords:Perilla frutescens, endophytic fungus, chemical constituents, bioactivity,

摘 要 ：采用硅胶柱层析和Sephadex LH-20凝胶柱层析等分离方法，从紫苏内生真菌Aspergillus sp.12Y03发酵产物中分离鉴定了10个化合物，经现代光谱学技术鉴定为：环-(脯氨酸-甘氨酸)(1)、环-(脯氨酸-丝氨酸)(2)、环-(丝氨酸-4-OH-脯氨酸)(3)、环-(丙氨酸-4-OH-脯氨酸)(4)、环-(苯丙氨酸-甘氨酸)(5)、环-(丙氨酸-甘氨酸)(6)、亚油酸(7)、α-亚麻酸(8)、cerevisterol(9)和22E,24 R-5α,6α-环氧麦角甾-8(14),22-二烯-3β,7α-二醇(10)，均为首次从该菌种中分离得到，化合物3和4具有中等强度的海虾致死活性。

Abstract:Ten compounds were isolated from the culture of an endophytic fungus Aspergillus sp.12Y03 in Perilla frutescens.Their structures were elucidated by spectroscopic methods as cyclo-(Pro-Gly)(1),cyclo-(Ser-Pro)(2),cyclo-(Ser-4-OH-Pro)(3),cyclo-(Ala-4-OH-Pro)(4),cyclo-(Gly-Phe)(5),cyclo-(Gly-Ala)(6),linoleic acid(7),α-linolenic acid(8),cerevisterol(9) and 22E,24R-5α,6α-epoxyergosta-8(14),22-diene-3β,7α-diol(10).All compounds were isolated from this fungus for the first time.Compound 3 and 4 was shown to be moderate active toward brine shrimp larvae.

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