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作 者 ：王日升 1,2，张 曼 1，李 猷2,3，黄凤婵 1,刘文君 1，郭晓燕4

期 刊 ：西北植物学报 2012年 2期 页码：425~430

关键词：抑制差减杂交；植物；抗病虫；表达序列标签；

Keywords:suppression subtractive hybridization, plant, ESTs of defense disease and pest, expressed sequence tags,

摘 要 ：抑制差减杂交技术广泛应用于植物抗病虫（真菌、细菌和线虫）机理研究。本文在归纳出植物抗病虫SSH文库中ESTs的总体情况之基础上，重点分析了表达频率高的抗病、防御和信号转导基因，并展望了SSH的发展前景，有利于认识植物抗病虫分子机理的普遍规律和推广应用该技术。

Abstract:Suppression subtractive hybridization technology has been widely applied in the mechanism research of plant defense disease and insect pest,especially for fungi,bacteria and nematodes.The overall condition of ESTs from suppression subtractive hybridization libraries for plant resistance to disease and insect pest were generalized,and some highly expressed genes such as resistance genes,defense genes and signal transaction genes were analyzed emphatically.Finally,the future of SSH technology were reviewed.These helps to understand the universal rule of the mechanism of plant defense disease and insect pest,and enhance the application of suppression subtractive hybridization technology.

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