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Genetic Diversity of Six Corylus Species in China Detected with Microsatellite Isolated from Corylus avellana

欧洲榛微卫星对我国榛属种质资源的分析


摘 要:利用多态性高、重复性好的11对欧洲榛微卫星引物对榛属6个近缘种的34个DNA样本进行扩增,共获得81个等位基因,每个座位的等位基因数在4~13之间,平均数目为7.36个,位点的多态信息含量(PIC)介于0.47~0.86,平均为0.73。PCR扩增产物的DNA测序证明欧洲榛SSR基因座位中微卫星序列在6个种内的保守性,而在CAC C28位点,一个被忽略的短三碱基重复序列也表现出长度多态性。另外,对于具有商业潜力的平榛、毛榛、川榛3个种的遗传多样性分析表明,平榛的遗传多样性最高,为0.59。研究结果表明欧洲榛微卫星是用于榛属资源保护、品种改良以及种间遗传图谱构建的有力工具。

Abstract: Diversity of 6-Corylus-species distributed in China was analyzed by 11 SSR markers isolated from C. avellana. A total of 81 alleles were amplified by 11 high informative SSR primer pairs from 34 DNA templates of the accessions tested. Number of alleles per locus ranged from 4 to 13 with an average value of 7.36. Average value of polymorphic information content (PIC) was 0.73,ranged from 0.47 to 0.86 Sequencing the PCR amplified DNA showed there existed microsatellites of C.avellana in 6-Corylus-species. Other short tri-nucleotide repeat sequence also exhibited variable in repeat numbers at CAC C28 locus. In addition,the average Nei‘s gene diversity index was 0.59 for all the loci of three species, including Corylus heterophylla,Corylus mandshurica and Corylus heterophylla var. sutchuenensis, which were of potential commercial value. This study demonstrated that the silver-staining SSR was a useful tool for genetic diversification, source conservation, variety identification and construction of molecular map among species of the genus Corylus.


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