研究芒果壮铗普瘿蚊不同程度为害下寄主植物叶片中硝态氮、可溶性糖、可溶性蛋白质、叶绿素含量以及植物保护酶系——超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的变化。将叶片受害程度划分为4个等级:1~50,51~100,101~200,>200(每叶片虫瘿数),并以无虫瘿为害的叶片为对照组(CK)。结果表明:可溶性糖与硝态氮含量在各个等级之间均没有显著的差异; 可溶性蛋白和叶绿素a、 叶绿素b含量各为害等级叶片均显著低于CK; 而类胡萝卜素含量,高密度为害等级(101~200,>200)显著高于其他各组及 CK。植物保护酶系测定结果显示:CK组活力最低,>200等级SOD,POD,CAT活力均高于其他各组; 其中, 51~100与>200等级SOD活力显著高于其他等级; 为害等级量重的(>200)POD和CAT活力显著高于其他等级; 而1~50等级显著低于其他等级。因此,入侵害虫芒果壮铗普瘿蚊的不同为害程度直接影响植物的光合作用及有关营养物质; 随着为害等级的加重可导致植物保护酶系的活性增大。
Procontarinia robusta, found in Xiamen downtown in 2000, is an insect harming mango trees. In this paper, we report the physiological and biochemical changes of the mango tree when infested by Procontarinai robusta. The the physiological and biochemical indices included total soluble protein, soluble sugar, nitrate nitrogen and pigment contents, as well as nitrate reductase (NR), superoxide dismutases (SOD), peroxidases (POD) and catalase (CAT) activities. The leaf damage was classified into four levels with 1-50, 51-100, 101-200 and >200 galls/leaf, respectively. The uninfected leaves served as control (CK). The results showed that there were no significant differences in soluble sugar and nitrate nitrogen content among levels. The infested leaves had significantly lower chlorophyll a and b content and soluble protein content than the CK. The levels of 101-200 and >200 galls/leaf had significantly higher carotenoid content than the CK. SOD, POD and CAT activities increased in the infested leaves. SOD activity in 51-100 and >200/leaf levels was significantly higher than the other levels. The level of >200 galls/leaf exhibited significantly higher POD and CAT activity than the other levels, while the level of 1-50 galls/leaf had lower POD and CAT activity than the other levels. Therefore, P. Robusta would have direct effects on plant photosynthesis. The protective enzyme activities also increased with the infestation degree.
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