作 者 :李伟;;陈晓阳
期 刊 :林业科学 2010年 46卷 12期 页码:42-48
关键词:欧洲山杨 ;叶片衰老 ;光周期候选基因 ;单核苷酸多态性 ;关联分析 ;
Keywords:Populus tremula , leaf autumn senescence , candidate genes , single nucleotide polymorphism , association analysis ,
摘 要 :
利用自然更新的欧洲山杨群体,以光周期途径基因为候选基因,通过连锁不平衡作图,对候选基因中能够引起氨基酸改变的单核苷酸多态性(SNP)与欧洲山杨秋季叶片衰老相关指标进行关联分析。结果表明: 光敏色素A中第291个核苷酸A和G的转换,导致了3个基因型叶片衰老指数有显著差异(P =0.018,经Bonferroni 修正后,P =0.04);光周期候选基因中的SNP、叶片衰老持续时间基因型值间无显著差异,SNP与叶片衰老持续时间之间不存在线性相关; 同样是光敏色素A中位于第291个核苷酸位点的3种基因型,其叶片衰老起始时间的基因型值差异显著,但是经Bonferroni 修正后的显著水平P >0.05,差异又不显著,反映了欧洲山杨秋季叶片衰老起始时间的复杂性。
Abstract:
In this study, association analyses between leaf autumn senescence and single nucleotide polymorphisms (SNPs) derived from amino acid change in the genes of photoperiod pathway of Populus tremula were conducted by linkage disequilibrium mapping (LD mapping) with naturally regenerated aspen populations. The results showed that nucleotide transition between A and G in the 291 nucleotide loci of Phytochrome A gene led to significant variation in autumn senescence index among the three genotypes of AA, AG and GG. The variance P -value between the three genotypes was 0.018 and the Bonferroni adjusted P -value became 0.04; No significant linear correlation was detected between all the selected SNPs in the candidate genes of photoperiod pathway and the senescence duration; Values of the same genotypes in the 291 nucleotide loci of Phytochrome A gene demonstrated significant variance in the starting day of senescence. But the Bonferroni adjusted P -value was more than 0.05, which indicated no significant variance existed between the senescence starting days of the three genotypes, which suggested the complication in regulating senescence starting day during autumn senescence in Europe aspen.
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