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作 者 ：戴莲韵 王学聘 杨光滢 张万儒

期 刊 ：林业科学 1994年 30卷 2期 页码：117-123

关键词：自然保护区；森林土壤；芽孢杆菌；苏云金芽孢杆菌；

Keywords:Nature Reserves, Forest soil, Bacillus, Bacillus thuringiensis,

摘 要 ：从大兴安岭根河潮查、带岭、千佛山、崂山、黄山、紫金山、武夷山和西双版纳八个自然保护区采集到0—5cm土层林下土壤样品129个,从中分离出大量的苏云金芽孢杆菌。研究表明:不同立地带森林土壤中芽孢杆菌的总数量、苏云金芽孢杆菌的出土率及占所分离芽孢杆菌的分离率间具有一定的差异,但它们与土壤含水量、pH、单一养分间无明显的相关性。我们还对所分离的苏云金芽孢杆菌所属亚种进行了初步鉴定,对六种有害昆虫进行了毒力测定。

Abstract:A number of isolates of Bacillus thuringiensis were obtained from 129forest soil samples at 0-5 cm depth collected from 8 Nature Reserves Chaocha, Dailing, Qiangfoshan, Laoshan,Huangshan, Zijinshan,Wuyishan,and Xishuangbanna) in China. The number of Bacillus and the rate of isolates(% )of Bacillus thuringiensis showed difference in forest soil of distinct Nature Reserves.Calculation of rate of Bacillus thuringiensis to Bacillus in these forest soils also showed difference.These differences have no correlation with soilmoisture,pH, and nurture of forest soil.The isolates of Bacillus thuringiensis were identified. The toxicities of isolates of Bacillus thuringiensis against 6 species insects were determined.

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