摘 要 ：利用江西的红豆杉(Taxus chinensis)、福建的南方红豆杉(Taxus chinensis var.mairei)以及四川的南方红豆杉(Taxus chinensis var mairei)的成熟胚为试验材料,成功地诱导出大量的愈伤组织。这3种材料愈伤组织的最高诱导频率分别为95.7%、98.4%、91.7%。细胞学和形态学的观察结果表明:在适当的条件下经过一段时间的培养,这些愈伤组织能够产生处在不同发育阶段的体细胞胚。上述3种植物材料每100块愈伤组织平均最多分别可产生127、91和33个体细胞胚。
Abstract:Lots of calli were. successfully induced by culturing the mature embryos of Taxus chinensis, Taxus chinensis var. mairei (Fujian) and Taxus chinensis var. mairei (Sichuan). The highest calli-inducing frequency was 95.7%,98.4% and 91.7% respectively. The cellular and morphological observation revealed that somatic embryos at different growth stages could be produced from the calli which were cultured for some time under appropriate culture condition. For the above three materials, the largest number of somatic embryos per 100 calli was 127, 91 and 33 respectively.