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作 者 ：杜学梅;李登科;王永康;隋串玲;樊保国

期 刊 ：园艺学报 2005年 32卷 3期 页码：496-499

关键词：枣；胚；组织培养；

Keywords:Chinese jujube, Embryo, Tissue culture,

摘 要 ：以‘六月鲜’枣为试材, 对花后12～82 d的幼胚进行培养。结果表明, 适宜于胚挽救的最小胚龄为花后50 d左右; 在胚形成前进行离体培养, 蔗糖浓度以7%为宜, 而胚形成后离体培养以5%蔗糖为宜; 暗培养可促进离体胚的生长发育, 且有利于生根; 植物生长调节剂及有机添加物极显著地影响着胚培效果。花后70～72 d胚龄的胚适宜培养基为1 /2MS + IBA 0.4 (mg/L, 单位下同) +BA 0.8 +LH 500 + GA₃5 + 5%蔗糖。胚培苗经扩繁后转入1 /2 MS + IBA 1.0 + IAA 0.06 + 2%蔗糖培养基上, 获得了健壮生根苗,生根苗移栽成活率达85%以上。

Abstract:The factors affecting embryo culture were studied in Zizyphus jujuba ‘Liuyuexian’. The youngest embryo saved successfullywas 50 days old. For the forming embryos, the op timal concentration of sucrose was 7% , for the formed ones was 5%. Dark treatment is beneficial to embryo sprouting, growing and rooting. There are extremely significance effects of plant growth regulators and organic substance on embryo culture. The media of 1 /2MS + IBA 0.4 (mg/L, the same below ) +BA 0.8 +LH 500 + GA₃ 5 + 5%
sucrose is suitable for culture of 70 - 72 days old embryo. The rootless seedling should be transferred to 1 /2 MS + IBA 1.0 + IAA 0.06 + 2% sucrose to induce normal root system.

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