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作 者 ：宋会访;葛　红;周　媛;王彩云

期 刊 ：园艺学报 2005年 32卷 4期 页码：738-740

关键词：桂花；离体培养；快速繁殖；

Keywords:Sweet osmanthus, In vitro culture, Rapid propagation,

摘 要 ：以桂花(Osmanthus fragrans Lour. ) 胚和新梢茎段为外植体进行了离体培养与快速繁殖研究,筛选出最适培养基——— (1) 胚萌发: MS +BA 1.00 mg·L ^{- 1} +蔗糖3%; ( 2) 新梢茎段启动培养: LMc +KT 8.00 mg·L ^{- 1}+NAA 0.10 mg·L ^{- 1} +蔗糖3%; ( 3) 继代增殖培养: LMc + TDZ 0.50 mg·L ^{- 1} +NAA0.10 mg·L ^{- 1} +蔗糖3%; (4) 生根培养: 1/2MS +NAA 2.00 mg·L ^{- 1} +蔗糖3%。采用腐殖质土为栽培基质, 移栽成活率可达80%以上。

Abstract:Using the embryo, stem-segment of new shoots as the exp lants, in vitro culture and high frequency propagation of sweet osmanthus were studied. The results showed that the optimizing media for various stages were as follows: (1) Initial medium for embryo: MS +BA 1.00 mg·L ^{- 1} + 3% sucrose; (2) Initial
medium of stem-segment for new shoots: LMc + KT 8.00 mg·L ^{- 1} +NAA 0.10 mg·L ^{- 1} + 3% sucrose; (3) Clump shoot regeneration medium: LMc + TDZ 0.50 mg·L ^{- 1} +NAA 0.10 mg·L ^{- 1} + 3% sucrose; ( 4) Rooting medium: 1 /2MS +NAA 2.00 mg·L ^{- 1} + 3% sucrose. Using humus soil as culture substrate, the
survival percentage of plantlets could reach 80%.

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