Abstract:Effects of different light qualities on the root growth and development of grape test-tube plantlets ofwhich were capable of different rootingswere studied. The results indicated that all of treatments didn‘t initiate to root the first five days after inoculating. The rooting fastigium of‘Yatomi Rosa’and ‘Centennial Seedless’of easy rootingwas from the sixth day to the tenth day underwhite light, red light, yellow light and the darkness. And their root numbers reached the maximum under white light after inoculating for five days under green and blue light respectively, butwhen theywere inoculated beyond five days under green and blue light, their rooting obviously restrained. W ith p rolonging the inoculating time under four homochromous lights and darkness conditions, their root fresh mass decreased. The root length and fresh mass of‘Fujiminori’and ‘Autumn Royal’of difficult rooting treated for ten days under red, yellow light and the dark conditions reached the maximum, then with prolonging the inoculating time, their root fresh mass decreased. But their root fresh mass and root length decreased under green and blue lights. So the conclusion was drawn that the lonwave light was benefit to the root growth and development, but the short-wave light was on the contrary.