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Effects of Salt Stress on the Ions Uptake and Transport of Leymus secalinus Seedling

盐胁迫对赖草离子吸收和运输的影响



全 文 :!23" !3#
 Vol.23  No.3
! " # $
ACTA AGRESTIA SINICA
   2015$  5%
  May.  2015
犱狅犻:10.11733/j.issn.10070435.2015.03.012
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?íî¤NaCl(0,100,200,300,400,500,600,700mM),Na2CO3 j NaHCO3(0,50,75,100,150,200,300,
400mM)<©ZN]„…(犔犲狔犿狌狊狊犲犮犲犾犻狀狌狊)fyÞ5W4#,:;ÌÍ|‹ŒUS‚r¯"j‚rIp×ñ+
^

JK¶·N]I3Õñ5

€a+›
:NaCl,Na2CO3 j NaHCO3 4#¢,N]„…ˆùI Na+¯"vÑÞK
2I¯"
,Cl-¯"˜K2Ñވù,ˆùI@ONa+ô."ՇeIùúæùà。N]„…f300mM NaClP
‹ýÕÖ
,Na2CO3‡eƒÞ150mMvP:;K+jCa2+IÒD:lONa+jClÒ Iç,NaHCO3 ‡eÑÞ
200mMŽ,K2Na+jClÒ ¯"ùú,ý4#Ž~。"ՇeIùú,Na/Ktùú,-K2Iùú"4ވù,
!›N]I3ÕÖµ˜:;ˆù@ONa+ƒK2+^,Ïæ­Ó6†}Na+89:

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:Q945.78    >?@AB:A     >CD=:10070435(2015)03051007
犈犳犳犲犮狋狊狅犳犛犪犾狋犛狋狉犲狊狊狅狀狋犺犲犐狅狀狊犝狆狋犪犽犲犪狀犱犜狉犪狀狊狆狅狉狋狅犳
犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊犛犲犲犱犾犻狀犵
DULixia,ZHUHuisen,DONGKuanhu,FANPengpeng,SUNJianping
(ColegeofAnimalScienceandTechnology,ShanxiAgriculturalUniversity,Taigu,ShanxiProvince030801,China)
犃犫狊狋狉犪犮狋:Astudyonionsuptakeandtransportinthedifferentpartsof犔犲狔犿狌狊狊犲犮犪犾犻犿狌狊seedlingwas
conductedunderthedifferentconcentrationsofNaCl(0,100,200,300,400,500,600,700mM),Na2CO3and
NaHCO3(0,50,75,100,150,200,300,400mM)stressinthegreenhouseexperiment.Theresultsshowed
thattheNa+ofacrialpartwashigherthanroots;thechangeofCl- wasoppositetothatofNa+ under
NaCl,Na2CO3andNaHCO3stresses.TherestrictingNa+capabilityofrootincreasedwiththeincreasesof
NaCl,Na2CO3andNaHCO3concentrations.Seedlingsof犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊couldgrownormalywiththe
NaClstressoflessthan300mM,butitwasinhibitedatmorethan200mM NaHCO3stress.Under150
mMNa2CO3stress,seedlingof犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊accumulatedK+andCa2+inordertoinhibittheincreases
ofNa+andCl-.Na/KratioincreasedwiththeincreasesofNaCl,Na2CO3,andNaHCO3concentrations,
buttheincreasingamountofNa/Kinrootwerehigherthaninacrialpart.Therefore,thesalttolerant
mechanismof犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊wasthatrootsysteminhibitedthetransportationofNa+.
犓犲狔狑狅狉犱狊:犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊;Saltstress;Ionsdistribution;Selectivetransportations
EFGH
:20140804;IJGH:20141202
KLMN

r0(-„Õ·¿¥
(201403110132);¥à®2Ê¿¥(2006056)ÀÁ
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:6x×Authorforcorrespondence,Email:dongkuanhu@126.com
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؁ƒÕDI¥I

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äØÃ(ÕD4ŽI2/3


ÕDmVnJ_ Na+,Cl-,CO2-3 ,HCO-3 j
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Þ17:00-20:00Ž5W。e…Å}2~3ŠŽ‘©
ˆ…606。q…2A%¢¤‡e_100,200,300,
400,500,600,700mM I NaClønj‡e_50,
75,100,150,200,300,400 mM I NaHCO3 j
Na2CO3 5W4#,Շe‘È’¥ˆ‡e–e5
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1.4 m®e@1VW
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ŒŽˆ Cl- ‚rI¯",°¤ AgNO3 wˆ³
ˆ
[17]。
Ca2+ˆ:ž&F»Þ›ÏÝ(580℃)Ý\,°
¤ÑÞ½¿wˆCa2+¯"。
ž&<K+jNa+p×ñ+^j@Na+ô.:
SNa,K(+^)=Na(2/ˆ)/K(2/ˆ)[18],SNa,K
+ÅNa+jK+fK2jˆùIp×ñ+^tu。
@Na+ô.= ˆùNa+¯"/K2Na+¯"[19]。
1.5 ijg
:;Excel2003jSPSS18.05jú¼JK

P¤Duncan5Wø~tS,Ó8G
HvPvð±{%©+Å。
2 mnU,c
2.1 犖犪犆犾€Á”*˜™€\¯•ˆ*åoÔ
Õ
"#NaCl‡eIùú,K2jˆù Na+I¯
"ùfœ…ùú

‰1A),Na+¯"fK2jˆù
üf100mMjCKÿœ‹ãRC,|‘‡eI
¯"vœ‹ÑÞ<à
(犘<0.05)。K2Na+¯"f
700mM NaClŽ}Øzà,ˆù Na+ ¯"f400
mMŽ}zà,400mM C700mM ghÿœ‹ã
R
。NaCl‡ef400mMP†Ž,ˆùNa+¯"ù
úSR

ç"œ‹àÞK2

!›NŽˆù+,q
‰/I@Na+ô.。
NaCl4#,ˆù K+¯"Sƒ,f)A‡e
ghjCKãRv‹œ‹(‰1B)。K2 K+¯"
"NaCl‡eIùúß>ùú¢ƒI”•,f300
mMNaClŽzÑ,g¢。
K2Ca2+¯""# NaCl‡eIùúfƒ,
‡ef400mMg¢ân†÷(‰1C),ˆùCa2+
¯"" NaCl‡eùúß>ùú¢ƒI”•,‡
e300mMNaClŽzÑ。
115
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<1 犖犪犆犾€”*犖犪+、犓+、犆犪2+、犆犾-•ˆ*åo’)
Fig.1 ContentsofNa+(A),K+(B),Ca2+(C)andCl-(D)in犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊underNaClstress
•

‰8‹Œ4…wx+ÅãRœ‹
(犘 <0.05)。Œ
Note:Valueswithinacolumnfolowedbydifferentlettersaresignificantlydifferentat犘 <0.05.Thesameasbelow
  K2jˆù Cl- ¯"—\Sà(‰1D):"
NaCl‡eIùúK2Cl-¯"œ‹ùú,)‡e¶
CKhãRvœ‹(犘<0.05)。e NaCl‡eˆ;
200mMŽ,ˆùCl-¯"œ…ùú,ç¶CKãR
œ‹
(犘<0.05)。NaCl4#,K2Cl-Iç"
àވùIç"

2.2 犖犪犎犆犗3 €Á”*˜™€\¯•ˆ*å
oÔÕ
NaHCO3 4#,K2Na+I¯"—\‹›œ

‰2A),çâ‡ef300mMj400mMŽ¶CK
ãRœ‹
(犘<0.05);æˆù Na+I¯""‡eI
ùúf÷Ñ

vœ‹ÑÞ<à
(犘<0.05)。+›ˆ
ùx_zHƒýNaHCO3 4#ISÝ,<Na+I
看ÑÞK2

K2K+¯"f‡e150mMNaHCO3 QP
/✋—(
‰2B),150mM P†Û܍,j
CKãRœ‹。ˆù K+ ¯"ƒÞK2,uvf1
mg·g-1P。
  NaHCO3 4#,K2Ca2+¯"I—\‹Î|

‰2C),f‡e100mM}Øzà,g¢ƒ,300
mM‚ùú。ˆùCa2+I—\”•jK2¬Ù,ç
˜f75mM }zà,çˆùI¯"4ÞK2I¯
"

"NaHCO3 ‡eIùú,K2 Cl- ¯"ùú

‰2D),0.05);ˆùCl- ¯"çfzчej CK âã
R

ˆùCl-¯"Iç"4ÞK2Iç"。
³†

f NaHCO3 4#,K+,Cl-jCa2+I
¯"ù˜K2àވù
,Na+˜ˆù¯"àÞK2。
2.3 犖犪2犆犗3 €Á”*˜™€\¯•ˆ*åo
ÔÕ
¶ CK ¬ t,N ] „ … K 2 Na+ ¯ " "
Na2CO3 ‡eùúfùú(‰3A),‡ef200mM
¶<àãRœ‹

ˆù Na+I¯"" Na2CO3 ‡e
ùúf÷Ñ

)Ë5Na+I¯"vœ‹ÑÞ<à(犘
<0.05),çˆùIç"àÞK2Iç"。
215
!3# £•:Õ4#<2 犖犪犎犆犗3 €”*˜™犖犪+、犓+、犆犪2+、犆犾-•ˆ*åo’)
Fig.2 ContentsofNa+(A),K+(B),Ca2+(C)andCl-(D)in犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊seedlingunderNaHCO3stress
<3 犖犪2犆犗3 €”*˜™犖犪+、犓+、犆犪2+、犆犾-•ˆ*åo’)
Fig.3 ContentsofNa+(A),K+(B),Ca2+(C)andCl-(D)in犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊seedlingunderNa2CO3stress
  Na2CO3 4#,K2jˆùK+¯"—\f)
‡ehÿ›œÎ|

‰3B),nâ‡eK2K+¯
"vàވùIç"

¶CK¬t,K2Ca2+¯"âùúI”•(‰
2C),‡e_200mM P¢Û܁ƒ。ˆù8Ca2+
ç">ùú¢ƒ

‡e100mM }Øzà,}
4.2mg·g-1。
K2Cl-¯""Na2CO3 ‡eIùúœ‹÷Ñ
315
! " # $ !23"

‰2D),‹Œ‡eIç"jCKv+,œ‹
ãR
(犘<0.05)。ˆùCl-Iç"f150mM P
jCKãR‹œ‹,200mM Žßöùú,¯ "}
Øzà

}15.5mg·g-1,g¢。
2.4 7€”*˜™ÁNa/K“šNa+›Óo
ÔÕ
ž62å5INa/Kt˜ž&3ÕI~Vû
ü

N]„…f NaCl4#¢,K2jˆù Na/K
tù"NaCl‡eIùúfùà(+1),-‡e4Þ
300mMŽ,K2jˆùI Na/Kt¶CKÿœ‹
ãR

!›NaCl‡ef300mM P,N]„…‹
ÔýØÕIED

K2Na/Ktf‡e700mMŽ
}Øzà

tðÑ}140,-ˆùI Na/K t#S
à

‡ef600mM Ž}Ø155,!›fчeI
NaCl4#,Na+Iç"µµàÞK+Iç",
-Ï@ONa+֎Ú,‡ef300mM P†+,q
S/I@Na+ô.。
Na/Cat"# NaCl‡eIùúfùà,-K
2Itðf‡e200mM Ž¶CK+,qœ‹ã
R

ˆùItðf‡e300mM +,qœ‹ãR。
¬Œ4#NO

K2 Na/KjˆùI Na/KJ*
tNa/CaÑq16.4j14Y,!›ýØ4#¢K+
Içûe6K+’ëIûeùú。
¶CK¬t,NaHCO3 4#¢,K2Na/Ktf
‡e200mM Ž+,qœ‹ãR,ˆù Na/Ktf
nâ‡ev¶ CK ãRœ‹,@ Na+ ô.#¶
CKvãRœ‹,!›fNaHCO3 ‡e50mMN]
ˆùÛÛÜXO Na+ƒK2+^。Na/CatfK
28I—\/âÎ|

-tðS4

æˆùI Na/
Cat" NaHCO3 ‡eIùúfùà,5¥z!›
ˆù<Na+I@O。K2jˆùI Na/Ktvà
ÞNa/CaItð,!›N]ýØ4#¢,K+Iç
"V4ÞCa2+。
Na2CO3 4#¢,K2jˆùI Na/Kù˜"
‡eIùúfùà

-K2 Na/Kt¶CKf300
mM+,ãRœ‹,ˆù Na/Ktf100mM Žé
+,œ‹ãR

@Na+ô.f50mM ¶CKãR
œ‹

-Øчe
(300~400mM)@Na+ô.ÛÜ


¶CKãR‹œ‹,!›fчeˆùI@
Na+ô.,£ Na+à"ƒK2+^,ÏæÔD
2.

K2jˆùI Na/Cat”•j Na/Kt¥
Š

!›ˆù<Na+I0Ó,XO‰Ca2+Iç,æ
K2Na+I†‡,é)‰Ca2+I¯"。
SNa,K=Na(2/ˆ)/K(2/ˆ)˜N]< Na,K
Ip×ñ+^tæ

|tðà

!›K2<NaIp
×ñà

nýÔDà

- NaCl4#¢SNa,Kf300
mMPânùú,300mM P†Û܍,Na+ô.I”•¥Š;æNaHCO3 6Na2CO3 4#
¢
,SNa,K"‡eIùúfƒ,!›K2< KIp
×ñà

@O‰Na+I+^。
3 œ
ParidajDas[20]zqž&3ÕýOg¥Û˜]
Oˆ<Na+I0Ó,P†‡|ƒ2.I}+。?Ì
Í8
,NaCl4#N]„…,Na+ K2¯"f NaCl
700mMŽ}Øzà,ˆù8¯"f400mM Û}
Øzà

çg¢ˆù Na+ ç"›œàÞK2;
NaHCO3 jNa2CO3 4#¢,nâ‡eù˜ˆù
Na+ç"àÞK2Iç",Na+ô./,@O‰ Na+ƒK2+^。tzoulakis[21]<àá»`IÌ̀a¥Š,-­“

[22]
<4EIÌÍ+›

¬ŒÕË5

K2I
Na+¯"_ˆùI1.5~2.3Y,›œÑވù,ý
ø_4EIˆùPÿ›œâîÕJx¤

nP‹Œ
ž&3ÕIÖµ‹Œ

N]˜:;³·ÕJ‚rƒ
K2+^

Wà"INa@Ofˆù,†‡|IÔD

çâe4#/eˆ;ˆI]Oô.Ž


ˆåINa+u›œƒ4†U}%,Š£K28Na+
¯"ãÆ÷

à"€a{“Õ4#()ž62å5I
Na/Kt˜ž&3ÕI~Vûü,#˜)"ž&3
ÕñI~Vz{
[2324]。
Õ4#N]„…

ˆùjK
28INa/Ktv"ՇeIùúfùà,-ˆù
Iùú"àÞK2

ˆù8Na/KtzÑ_K28
I4.40Y,<5¥z!›ˆù@O‰ Na+ƒK2
+^

()K2SƒINa/Kt。
ž&3ÕñI/Æ

‹<+,f<Na+‚r0
ÓI/Æ

æç+,fÕ4#ž6ï2I‚r0
ÓPQJ{IÒDô.
[25]。Bauls[26]fäå8
Ì͒,Ca2+ôÛâ>4ƒ Na+jCl-ψù
ƒ2.I+^

àøGž&<Na+jCl-I0Ó¶
K+jCa2+I0Óðâ&ñ,c"#2å Na+¯
"†÷K+jCa2+¯"°。Ì͒,

Õ4#K2 Na+jCl-ù˜"‡eIù
úfùú

æK+jCa2+fƒ‡eŽ—\v„Æ,Ñ
‡e
(NaCl400mM,Na2CO3200mM,NaHCO3200
415
!3# £•:Õ4#mM)Û܍;ˆù8INa+jK2—\”•¥
Š

-K+I—\‹›œ,Ca2+>ùú¢ƒ,Cl-œ
‹ùú

<#{“‰ Na+jCl-I0ÓXO‰6Õ
։K+jCa2+I0Ó。
q1 7€Á”*˜™€\¯犖犪/犓犖犪/犆犪“1ë0š犖犪+›ÓoÔÕ
Table1 EffectsofsaltstressontheratiosofNa/KandNa/Cainthedifferentpartsof犔犲狔犿狌狊狊犲犮犪犾犻狀狌狊seedlings
andtherestrictingNa+capabilityofrootsystem
K2Na/K
Na/Kradio
ofshoot
K2Na/Ca
Na/Caradio
ofshoot
ˆùNa/K
Na/Kradio
ofroot
ˆùNa/Ca
Na/Caradio
ofroot
@Na+ô.
Restricting
Na+capability
SNa,K
=Na(2/ˆ)
/K(2/ˆ)
NaCl‡e
NaCl
concentration
/mM
CK 1.22±0.11d 1.16±0.03b 2.67±0.19d 0.85±0.16d 0.66±0.07b 0.45
100 20.86±0.92d 3.43±0.16b 30.62±0.82d 1.40±.21d 0.27±0.06c 0.58
200 43.65±7.47cd 9.43±0.38a 34.22±1.56d 1.53±0.14d 0.25±0.03c 0.47
300 35.44±3.88cd 8.92±1.69a 45.02±1.86d 3.87±0.22c 1.38±0.07a 0.35
400 80.97±2.2bc 9.16±0.56a 91.06±4.35c 11.79±1.14b 1.49±0.10a 0.25
500 116.67±26.94ab 8.55±1.04a 102.12±9.39bc 11.64±0.33b 1.43±0.14a 0.28
600 90.04±16.11b 7.37±0.83a 154.58±18.42a 11.02±0.58b 1.52±0.18a 0.18
700 139.55±25.57a 9.23±0.81a 134.37±28.79ab 14.49±1.24a 1.36±0.14a 0.37
NaHCO3‡e
NaHCO3
concentration
/mM
CK 1.22±0.11c 1.16±0.03bc 2.67±0.19d 0.85±0.16e 0.66±0.07b 0.45
50 1.56±0.18bc 1.20±0.18bc 6.46±0.56bc 1.99±0.13cd 1.61±0.1a 0.25
75 1.75±0.27bc 1.48±0.03ab 7.44±0.62b 1.91±0.3d 1.53±0.2a 0.21
100 1.71±0.12bc 1.06±0.07c 7.61±0.5b 2.23±0.25bcd 1.57±0.27a 0.43
150 1.79±0.34bc 1.26±0.05abc 7.89±0.49b 2.56±0.17bc 1.64±0.07a 0.23
200 2.61±0.51ab 1.59±0.03a 4.94±0.48c 2.81±0.17b 1.72±0.13a 0.22
300 3.35±0.52a 1.41±0.17abc 6.56±0.82bc 3.53±0.26a 1.57±0.1a 0.34
400 3.26±0.58a 1.54±0.24ab 13.57±1.26a 2.50±0.13bcd 1.65±0.22a 0.32
Na2CO3‡e
Na2CO3
concentration
/mM
CK 1.22±0.11c 1.16±0.03b 2.67±0.19d 0.85±0.16e 0.66±0.07c 0.45
50 1.57±0.03c 1.50±0.03b 6.37±0.85cd 2.22±0.29cd 1.56±0.21a 0.24
75 1.56±0.06c 1.48±0.14b 6.40±0.17cd 2.56±0.34bc 1.61±0.13a 0.22
100 1.67±0.91c 1.68±0.29b 7.58±0.45c 1.70±0.08d 1.46±0.19ab 0.22
150 2.98±0.07abc 1.14±0.04b 7.45±0.84c 1.91±0.14cd 1.66±0.13a 0.21
200 2.38±0.35bc 1.53±0.09b 10.40±0.75bc 2.62±0.14abc 1.33±0.12ab 0.5
300 4.56±1.37a 3.60±0.67a 13.41±2.04a 3.16±0.4ab 0.97±0.21bc 0.5
400 4.30±0.92ab 3.72±0.45a 12.99±2.91ab 3.37±0.38a 0.97±0.24bc 0.24
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