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作 者 ：郭国赟, 孙润广, 郭琦, 张小飞, 窦佩娟

期 刊 ：植物科学学报 2012年 30卷 4期 页码：423-430

关键词：滑菇；滑菇多糖；热水提取；结构；

Keywords:Pholiota nameko, Pholiota nameko polysaccharides, Hot water extraction, Structure characterization,

摘 要 ：利用传统热水浸提法提取滑菇粗多糖(wPNP),进而用季铵盐沉淀法和Sephadex G-150凝胶色谱柱层析对wPNP进行纯化,得滑菇多糖wPNP-a1。以高效液相色谱(HPLC)、傅里叶变换红外光谱(FTIR)、气相色谱(GC)、核磁共振(NMR)、环境扫描电子显微镜(ESEM)和原子力显微镜(AMF)等方法分析wPNP-a1的结构,结果表明,滑菇多糖wPNP-a1的分子量为419310 Da,具有一般类多糖的特征吸收峰,糖苷键为β-型,不含乙酰基,由木糖、甘露糖、半乳糖和葡萄糖组成,其摩尔比为3.91∶2.77∶1.91∶1;wPNP-a1的分子间排斥力较大,分子间吸引力较小,在云母表面呈直链状分布,链宽23 nm左右,链高0.7~0.8 nm。

Abstract:Water-soluble crude polysaccharide (wPNP) was extracted from cultured mycelium of the fungus Pholiota nameko by hot water extraction.The extraction rates of polysaccharide were 2.463%.The content of total polysaccharide was determined by the phenol-sulphuric acid method,and the value was 64.98%.The crude polysaccharides were purified by CTAB and Sephadex-150 in sequence,with the obtained one designated as wPNP-a1.The structure of wPNP-a1 was analysed by HPLC,FTIR,GC,NMR,ESEM,and AFM.The results showed that the average molecular weight of wPNP-a1 was 419310 Da.It had the general characteristics of polysaccharide absorption peaks,β-glycoside linkage,and no acetyl.The GC analysis indicated wPNP-a1 was composed of xylose,mannose,galactose,and glucose in molar ratios of 3.91∶2.77∶1.91∶1.The repelling forces between the molecules were stronger than the attractive forces. The wPNP-a1 formed a linear chain on the surface of mica,the width was 23 nm,and the height was 0.7-0.8 nm.

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