作 者 ：曾艳;苏智先;邹利娟;吴庆贵;毛艳萍;程昌凤

期 刊 ：武汉植物学研究 2010年 28卷 2期 页码：218-223

关键词：强德勒红心柚；离体培养；丛生芽增殖；

Keywords:Citrus grandis Osbeck cv.Chandler, Culture in vitro, Bush-bud regeneration,

摘 要 ：

Abstract:The cotyledons,cotyledonary nodes,epicotyl segments,and stem segments with lateral buds of aseptic Citrus grandis Osbeck cv.Chandler seedings were used as the explants to study the process of tissue culture rapid reproduction.Results showed that the cotyledonary nodes were the best explants of bush-bud induction(induction rate 100%).The best medium for inducing bush-bud was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+sucrose 30 g/L+activated carbon 0.4 g/L,the coefficient of proliferation reached 11.2.The optimum medium for shoot rooting was 1/2MS+NAA 0.5 mg/L,which resulted in a rooting rate of 100%.Fifteen days after transplanting,100% of the plantlets survived.