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作 者 ：郭晓芳;严海燕;何骥;李立家;陶勇;曹进京

期 刊 ：武汉植物学研究 2006年 24卷 1期 页码：22-26

关键词：ABI3；FUS3；LEC2；Northern杂交；原位杂交；

Keywords:ABI3, FUS3, LEC2, Northern hybridization, In situ hybridization,

摘 要 ：ABSCISICACID-INSITIVE3(ABI3)、LEAFYCOTYLEDON2(LEC2)和FUSCA3(FUS3)转录因子在种子发育过程中发挥着重要的调控作用。采用Northern杂交技术,用拟南芥ABI3保守的B3结构域部分序列作为探针分别与花生根、茎、叶、子叶RNA进行了杂交,同时也对花生根、茎、叶、子叶(含胚)组织切片进行了原位杂交,结果均显示只有在花生的子叶和胚中有杂交信号出现,表明花生中可能存在ABI3、FUS3和LEC2的同源基因,且它们只分布在花生的子叶和胚中。

Abstract:Transcription factors ABSCISIC ACID-INSITIVE3(ABI3),LEAFY COTYLEDON(LEC2)and FUSCA3(FUS3)play important roles during seed development.The RNA extracted from peanut root,stem,leaf and developing cotyledon were hybridized by probe from the conserved B3 domain of Arabidopsis ABI3 protein.The micro sections of root,stem,leaf and developing cotyledon(including embryo) of peanut were also hybridized in situ with the same probe.The positive results of hybridization signals only in peanut cotyledon reveal that the homologous gene of ABI3,FUS3 and LEC2 may exist in peanut and they only distributed in the cotyledon and embryo of peanut.

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