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作 者 ：侯嵩生;李新明;尚廷兰;吴玉兰;李洪林

期 刊 ：武汉植物学研究 1990年 8卷 1期 页码：65-69

关键词：九连小檗；药根碱累积；细胞生长；蔗糖转化率；

Keywords:Berberis julianae Schneid, Jatrorrhizine accumulation, cell growth, Sucrose conversion,

摘 要 ：本文叙述了九连小檗植物细胞悬浮培养过程中,药根碱的累积和细胞生长与培养液中可溶性糖转化的关系.实验表明细胞培养过程中培养液的可溶性糖逐渐消耗,细胞生物量和药根碱量都逐渐增加,且细胞生长与药根碱累积的曲线几乎是平行的.然细胞生长速度较快,其生长速率曲线的峰形较尖陡.药根碱累积速度较慢,延续时间较长,其累积速率曲线的峰形较平缓.根据糖的消耗与细胞生物量增长和药根碱累积的关系,计算出蔗糖——细胞转化率为59%,蔗糖——药根碱转化率为3.3%.

Abstract:The relationship between cell growth and jatrorrhizine accumulation during the suspension culture of Berberis julianae Schneid cells was reported. In the B.julianae cell suspension culture the cell growth(dry weight per liter)increased gradually and reached a maximum in 16th day. The alkaloid content of the ceils increased nearly in parallel with cell growth curve, and reached a maximum in 19th day, and then tended to decrease. The cell growth rate (g/l/day) and alkaloid formation rate (mg/l/day) in suspension cuhurc of B.julianae cells are little difference. The rate of cell growth is quicker than that of alkaloid formation. The peak pattern of cell growth rate is sharper, it reached to peak on 12th day. The rate of alkaloid formation increased slowly and reached to peak on 16th day, it‘s peak form is showed much gently. When the cell growth decreased or ceased, the alkaloid formation is still proceeding. So that the time to peak of alkaloid formation rate is delayed 4 days. Based on the sugar consumption and cell growth the percentage of sucrose conversion was calculated by 59%.

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