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作 者 ：李双成;张玉;王世全;尹福强;邹良平;李平

期 刊 ：作物学报 2005年 31卷 08期 页码：1014-1020

关键词：农杆菌介导；短日豌豆花后特异表达基因PPF1；延缓衰老；叶绿素含量；光合指标；叶片衰老进程；

Keywords:Agrobacterium-mediated transformation, Post-floral-specific gene from pea(PPF1), Leaf-senescence delaying, Chlorophyll content, Photosynthetic index, Leaf senescence development,

摘 要 ：利用农杆菌介导法，把短日豌豆花后特异表达、具有延缓衰老作用的基因PPF1（post-floral-specific gene expressed in short-day-grown G2 pea）转入水稻，以期获得延缓衰老的转基因植株。约970块未成熟胚性愈伤组织与农杆菌共培养后，得到了约280块抗性愈伤组织，从中分化出抗性绿苗54株。PCR和Southern blotting检测结果表

Abstract:Premature senescence of leaf, usually results in too much abortive grains and lowing in seed-setting-rate, is a common problem for many plants and always led to great loss of production. In this study, the leaf senescence-delaying gene, PPF1, a post-floral-specific gene expressed in short-day-grown

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