Abstract:MicroRNAs (miRNAs) are endogenous non-coding single-strand molecules with 21-25 nucleotides in length, and they are widely existed in plants and animals. In plant, miRNAs have been confirmed that they negatively regulate gene expression negatively at the post-transcriptional level and play an important regulatory role in plant growth and development, and response to various biotic and abiotic stress. In recent years, miRNA has become the hotspot in modern molecule biology. In this paper, several methods for identification and charactrization of plant miRNAs were introduced, and further the processes and strategies for miRNA bioinformatics prediction were reviewed with emphasis, as well as investigation and analysis of some bioinformatics softwares. This paper will provide new ideas for miRNA identification in plant.