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期 刊 ：植物遗传资源学报 2014年 15卷 5期

关键词：华北落叶松；种子园；遗传多样性；微卫星(SSR)；

Keywords:Larix principis－rupprechtii Mayr., seed orchard, genetic diversity, microsatellite(SSR),

摘 要 ：华北落叶松是我国华北地区营造针叶树丰产林的首选树种，我国自上世纪60年代开始营建华北落叶松种子园，现部分种子园已建至第三代。但选优导致的种子园亲子代群体间的遗传多样性水平变化情况并不清楚，其分子水平的群体遗传基础也不明确，造成高世代育种及造林工作中种质资源不清等问题。本研究利用10对EST-SSR引物，分析了河北省龙头山林场华北落叶松良种基地种子园中一代、二代、三代优树群体的遗传多样性。每对引物的等位位点数为 2-5个不等。一、二、三代的平均有效等位位点数分别为1.9204个，1.81个，2.0693个，Shannon指数分别为0.7127，0.7052，0.7908，且群体中杂合子过量。结果表明，一至三代种子园群体遗传多样性水平没有明显的差异，子代群体与亲代群体间遗传分化较小，遗传变异主要存在于每代群体内，而非世代群体间。

Abstract:Larix principis－rupprechtii Mayr. is one of the most proper conifer species of conifer for forestation in north China. Seed orchards of Larix principis－rupprechtii Mayr. have been built since 1960s in China, and some of which have already contained three generations. However, lack knowledge of variations of genetic variationsdiversity after selection on molecular level causes some unclearness in breeding and forestation. 10 EST-SSR primers are used in this study to analysis the genetic diversity among three generations in the seed orchard in Hebei. Each primer has 2-5 loci. The numbers of average effective loci for the 1st, 2nd, and 3rd generations are 1.92, 1.81, 2.07. Shannon index are 0.7127, 0.7052, 0.7908. Heterozygote excesses in the population. The value of Fst is low, which shows that Genetic differences are mainly occurred in one generation rather than between generations.

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