Abstract:The somatic embryoids were initiated from cotyledon segments of two cultivars of Camellia sasanqua in vitro culture on the MS medium supple-mented with 6 -BA and NAA. It was obvious that the embryoids were formed directly from the surface of the cotyledons. When transferred onto a medium with 2 ppm BA, 0.5 ppm IAA, 0.2 ppm ABA and 500 ppm glutamine (Gin), the embryoids developed into complete plantlets. The growth regulator test demonstra-ted that GA3 had an inhibitory effect on formation of embryoids, but GA3 could stimulate shoot growth and root development of the mature embryoids. In subculture, a large number of secondary embryoids regenerated in 5-6 weeks. The plantlets were transferred to soil where they grown normally.