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期 刊 ：植物遗传资源学报 2012年 13卷 1期

关键词：葡萄种质；初级核心群；构建；

Keywords:grape genetic, primary core collection, construction,

摘 要 ：本文以国家果树种质郑州葡萄圃保存的867份栽培种质为材料，对47项表型性状进行了主成分分析。运用DPS3.01数据分析软件对数据进行标准化处理，采用欧氏遗传距离、离差平方和法进行种质初选。采用分组和逐步聚类法，分别以15%、20%、25%和30%的比例抽样，依次获得124、170、205和252份种质。通过对初选种质的遗传多样性指数、表型保留比例的分析，检验初级核心群的构建效果。结果表明，按种质类型分组，组内采用平方根策略、15%抽样比例获得的124份初选种质的表型保留比例和遗传多样性代表性均达到96%，表明构建的初级核心群对原始种质具有很好的代表性。

Abstract:Pricipal components analysis was conducted in 867 cultivated varieties from grape resources repository at Zhengzhou Fruit Research Institute of CAAS based on 47 phenotypic charactericses. The software of DPS3.01 was employed to standardize the data set and the germplasm were primarily selected with euclidean distance and ward method. Grouping and gradually cluster were used in this paper, each group 124, 170, 205 and 252 varieties were respectively obtained when the germplasm were sampled by square root strategy and ratio at 15%、20%、25% and 30% with the method of group-dividing and stepwise clustering. The representative of primary core collection was tested by using genetic diversity index, reserving phenotypes of characterics. One hundred of twenty-four varieties of grape primary core collection represent 96% of the whole germplasm when the germplasm was divided based on types and further sampled at ratio of 15% with the method of square root strategy in subgroup. The results showed that the primary core collection could well represent the genetic diversities of the whole collection．

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