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期 刊 ：植物遗传资源学报 2015年 16卷 2期

关键词：芦笋品种；人工海水；苗期胁迫处理；多元统计分析；耐盐性评价；

Keywords:Asparagus varieties, artificial sea water, stress at seedling stage, multiple statistics analysis, salt tolerance evaluation,

摘 要 ：利用浓度为25%的人工配制海水对25个芦笋品种进行了芽期和苗期的胁迫处理，调查发芽势、发芽指数、活力指数、胚根长、株高、茎数、茎粗、根鲜质量、根干质量以及根体积等10个农艺性状，以各性状耐盐系数作为衡量耐盐性的指标，利用多元统计分析方法对芦笋品种的耐盐性进行了综合评价。结果表明，调查的10个性状指标在不同品种间差异显著，因子分析将其集约于3个主因子上，即种子活力因子、根部因子和生长因子，主因子累计贡献率达82.2%。基于品种因子得分值进行聚类分析，25个芦笋品种可分为4类，即耐盐品种、较耐盐品种、中度耐盐品种和盐敏感品种。本研究建立的人工海水胁迫处理鉴定芦笋品种耐盐性的综合评价方法，可为芦笋材料耐盐性鉴定和耐盐育种提供理论参考。

Abstract:25% of artificial sea water was applied to stress total of 25 asparagus varieties at the germination and seedling stage. The salt tolerance of asparagus varieties was evaluated comprehensively by means of multiple statistics analysis based on the salt resistant index of 10 agronomic characters including trend of germination, germination index, vigor index, radicle length, plant height, stem number, stem diameter, fresh mass of root, dry mass of root and root volume. The result showed that 10 agronomic characters differentiated greatly in different varieties, and they could be characterized into three main factors by factor analysis，namely seed vigor factor, root factor and growth factor with the accumulative contribution rate of 82.2%．Cluster analysis based on the score vectors of the factors showed that the 25 varieties could be classified into four categories, including 6 of the best salt tolerant varieties, 4 of the stronger salt tolerant varieties, 7 of the moderately salt sensitive varieties and 8 of the salt sensitive varieties. Consequently, a comprehensive evaluation method of salt stress on asparagus varieties with artificial sea water was established in this study, which could offer theory references for salt tolerance identification and breeding on asparagus．

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