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作 者 ：张鉴铭 周建葵

期 刊 ：云南植物研究 1983年 5卷 04期 页码：1-3

关键词：702烟草冠瘿系；单细胞克隆；植株再生；

Keywords:single, cell, clonging, tobacco, tumour,

摘 要 ：用酶分离了根癌土壤杆菌702菌株诱发的烟草冠瘿及其畸胎瘤细胞系的原生质体被培养在修改的K及NT培养液中。当它们长成小细胞团时，转移到不加激素的NT培养基上筛选出T－DNA转化了的细胞形成的细胞团。这样，建立了单细胞克隆的702C烟草冠瘿系及其 702TC畸胎瘤系。经过15代继代培养，他们全部保持了他们原有的特征并且都含有 nopaline。用这种畸胎瘤的芽进行了分化根的试验，有少数芽长了根得到完整的植株。分别测试了植株的各部分，植株的叶及其冠瘿都含有nopaline，而根中未测出nopaline。可能根是由丢失了T-DNA的正常细胞长成的。也可能是它的基因未表达。

Abstract:The Protoplasts were isolated enzymatically from tobacco crown gall tu- mors and its teratoma cells induced by Agrobecterium tumefaciens strain 702. Small colonies ware derived from them by incubating these protoplasts in mo- dified K and NT liquid media. T-DNA transformed-cell colonies were selected by transferring them onto NT medium without any phytohormone. Then single cell cloning strain of tobacco crown gall turnor 702c and its teratoma 702Tc have been established. Through being subcultured 15 times they all maintain their inherent characrts and contain nopaline. Being carried on root differentiatc experiment with this teratoma shoots, roots grow from a few of them and intact plants were obtained. Examining each parts of the plants separately, nopaline was present in all leaves and crown galls but was not detected in the roots. The roots perhaps grow from normal cells losing T-DNA. Its genes also perhaps do not express.

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