免费文献传递   相关文献

Molecular cloning and expression analysis of sucrose synthase gene from Dendrobium officinale

铁皮石斛蔗糖合成酶基因的克隆及表达分析



全 文 :STUVWXÄÚYsZ[<[G¼m6
mno
R
#
ypq
R#X
#
rst
R#X
!
#
#AH
R
#
uvø
Q
#
8w
R
!Rg
©äÚL>¬ 5HôIJK
[
©äõ5Hôö¦÷øùúûüý5þ
#
©ä ÿ
YLMXMR%
Xg
]™Ú>¬ L!Aúû¬G
#
]™ #‹
QRRQMM%
Qg
©äøù¯Ô
‚;
%!

&
<[STUV
I&6(1,-5/13-)-2& WXÄÚYsZ>Cª¼m6 ! êƒ„STUV‘XÄÚiWXÄ ÚYe‰HG[y¼mµq¤,¥)  „… & s• @()7)W
_Ñï[WXÄÚYsZ
99
Z¬eJ
!

3A<83
Ñ
3>-
‡…©
!
<[STUVWXÄÚYsZOz
!
Xv[t™M^§#^’¼m’Ð
7CXR
r
!;8A@
±²¼m
!1T1<8>@-
/_6


†‡
&
Úà<[_STUVWXÄÚYsZ
!
sZ
GT?>
Oz
X _X_ PV!
WX
NM`sB
aMêTO11R%f
.eNLYNQL!
sZ`sBeJiýøÉÌ
K1Q( =&21G>JLQMLYN$$>JLQMLY\%
Z¬

`Mf!
ib£øÉÌ[
99
sZZ¬]
NMf
_

>Cª#˧#^’È+¼m±²
!
±²[NԒi.w¼mK|
ÙjC6A[·©ê
`X oRM
Q
c^[çv[
!
i¤,jC6A[·s£j0

†ˆ
&
Úà<[_STUVWXÄÚYsZ
!
>
±²K|Ùi¤,çvjC6A[·j0[çvÙd

$
‰Š‹
%!
STUV
&
WXÄÚY
&
sZ<[
&
¼m6
$
8Œ$f
%!XMRMRRRYMRL
$
ŽEq
%!
ËþRk§äùә€uv
$
€>
%!
!
eµÊ
!
üE
!
EF
!
üEã²f
!
‚&³ÙEÌÃ
Å¢[ƒ„NE
!-$
>š›
%!
ghi
!
üEƒ„ã
!A(&,RL`RRLLQLYM!-()*&]RYQ:G/I
!!
STUV
I&6(1,-5/13-)-2& êýø O%G#< 5G(( UVH‘÷ãÚ£ÉÌ ! ‚&6Q•Ëþ # ˜ @ # LV # *b # 7* # ¿À # úû # j܇Q R(  T ôǤ_ƒ„Æ.<årï)ê[klóK # ã mƋ # ÒnŒO‡ài® ! Yyèp×Ø # Š;± ÿ¹oP[E“ ! JpØp× # Mqr # M×.v [_‡AÄÇQ ! ‘XEê‚&eÚ6 X(  ÉÌ WXÄÚYp5s€TJ] R`LL ÷ ! 3kƒ„[Y ‘òÒ ! €T]ÉÌÿ~|žè X ¢_[ZNY ! WXZNY[WXÄÚYsZ¼myè9e±G  Q(  vhƒ„FtͳýøÉÌ K1Q(=&21G _( Guv L( wþx Y( # yz \( # }~ N( # ÑÒW `( ‡E ÌrXWXÄÚY99%
sZ<[K;

WXÄÚ
YJ[yWXôÏ[‰ŠYg|
!
vh{|)êW
XÄÚY[‚&àiJ]WX[}L…†
!
ê‘X
[ÄÚµqhÿ
!
à^WX#Ò¿¢ôÏ~*
RM(

ÇSK‡
RR(
]STUV÷Msr65ia_=·
[
_
¢Õ¬
!
ƒ„¼CÈ7ÌãzG‘XW³[
EF
!
0Œ¼.
,
WXрXœ•\XьX

W
X\·‚À
!
ãzÚ·[e܂§
!
‘X\·‚À

ƒ.‡
RX]„WUV÷Msria=·[9N
GnNw£µÀ_WXÄÚY[e
!
WXÄÚY
e[µÀaN_WX[6L
!
?#_ªÈ7Ì[
ãzёX[W³

£ƒ„ê#|䃄STUV
WXÄÚYsZ
1,3-
9s
#
àiGWXnf²
~*…V[[yƒ„µq¦§¥)
!
êµÀSTU
V‘X\·GãÌä·µq¨‘[ø_¤,¥)

! !
ÁÂi…©
! !`
ÉÌ
2n“ŒVÐéUV¢Éè˜IR99R%
sZ[†

A;>?@-?
˜%[
3?>V%(V V4%(
6
3?>
µçÛ÷;µSTUV[
3?>!

J(%I()20[T?>PçÛ÷;PT?>!2“J(0
˜
%[µM%çÛ÷C
3?>
#˵M%

ì)
@()<
7)W99sZ[A‡)°!®“V`_ m QM 0!LQ m
QM 0!\X m R I)%!\X m RM I)
KŠ·
R
Ùz
-QQN-
{
QY
ï{
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!>V%&! XMRR
êNRQ PV
[t™
!
Xt™<[·
8=TR`_
!
‘<[‰_ãN&e  ì) Qq3>- çÛ÷
*n§ôäã̘%
%
&
GÑï[
IR99S
Ø6eJ
!
á/
X
Ù±G•
Ì
,JX!@AA>>AA@>>@A&JQ!AA<
>>AA@>A>>@
ì)çÛ÷Ȥ
!

Šš
83
µ!
•Ì
JX
GçÛ÷r[
/42V%I(%
#Ë{
R
‹
83!
µ\Ôeü¬
,`_ m L I)!XM

O+
mQM0!YQmQM0!\XmRI){
R
5
83
äÌ
R
!
C
ê{
X
‹
83
µ[»ˆ
!

JQ
G
))2(%V%I(%
ê•Ì
!
µ\Ôeü
¬
,`_ m L I)!QM
O+
mQM0!YXmQM0!\XmRI)
L
!
C
#Ë/_6
!
†
_
YRM PV
[t™
!
ü_¢
!
<[&e

ì)
Lq3>-
çÛ÷
n§ôäã̘`_ m QM 0!LN m QM 0!\X
m X I)%!\X m RM I)
{ R 5 83 äÌ R ! C ê { X ‹ 83 µ\[»ˆ !  JX G ))2(%V%I(% ê• Ì ! µ\Ôeü¬ ,`_ m L I)!QM O+`_ m QM
0!LN m QM 0!\X m X I)%!\X m RM I)
L ! C # Ë/_6 ! †_ R NML PV [t™ ! ü_¢ ! <[& e  2“ T?>=>? ›s#ËeJŒw  !` !8-AÈ+¼mÚÿ[1Ü
ì)Ñ͆[STUV
IR99R
sZeJ
!
á
/KŠOz[•Ì
!
>]•ÌhŒ65a_
F1!
ÑC:1YyI-ÑAµhs
, 11J?/2
, > @@@@ >@@>>@@>@>@>>>A@@A@>& 11< 3+#/
, @ A@>@ @>AAA@@@>>AA<
A>AAA>
XKŠ[Ozt™<[·Úÿ
8->1c_
!
X†[
8->1c[»G
8-A¼mÚÿ
#Ë{Yy
!Q\ m
µ\ 
!RgXf
x€Xq‡/_
R&¾ÊJg^

g^äÌ2“
A_ &0(
ôw
!RY
m
 µ!
XôwäÌ
RM
!
C
aÒ<
RMM
!
C7CXR
SÚî·¸r
!
Ž_è3
QM I)!_X m

_L 0!
Ø
èŽ_
X I)!

N`M
!
CC7
÷Ms
!Q\ m
P÷
M
R #!
]\èövû[
C7
x€÷Ms_÷M

‘H’’!w¢·RMICC7÷MsW)LMCSRW$) LM *-C
SR
%
r
!Q\ m!XMM %-I)
SR
¬÷M<
;
YMM
MgY jMgN&>
;8A@

RgM
II/&-C
SR
!7
{Ya
!
:I“’÷M
L #
XJ°[
’<
RX MMM %-I)
SR

L I)
¾(_ó
!

RMM
!
C
v’6
!
ŸOkF
!

RMM
!
CX o1T1
_€./
<
!
ߖ
!
”6r•”
RM I)!
€Q]~U
RX MMM %
-I)
SR

L I)!
Ió·”|Ps[á1r!Ø6_ó“•
1T1<8>@-
q‡/_6

#
0Œi6
# !`
STUVWXÄÚYsZ[Oz<[
# !` !`
STUV6
3?>

!

A;>?@-?
3?>
µçÛ÷µSTUVC…°t[6
3?>!
͓ + B ç v 6  ß
>I(I 7/0G()G(0
@()(^4)2VIR99S%
sZ[Ø6
t™[±GKŠ
!
STUV[
GT?>
껈
!

JR!3R
ÀC±G•Ì#ËKŠ
!83
KŠ0
Œƒf
X!
KŠK
R
Ùó—
#
±GŸ
!
§8©ê
NMM
PV
[Ùd

ì)
A<>
<[©
!
XKŠäÌ<[·
8->1cÚÿ_
!
m pÑ˜v™4¶
!
34‘
ëH’’#Ë 83 ÕÔ ! ‰_ãN&e  & e0Œ¼. !83 KŠäÌ[t™§8ê NRQ PV! X™eJ#Ë 7&02

!
0Œw½eJi
@()7)W r[ K@=&21G [Z¬ê `Xf! i R)-6B -_QN- { QY ï{ — XMRR ÷ _ ´ !!!!!! ! ! ! ! ! !!!! ! ! ! ! U/&gQY! ;004(!>V%&! XMRR -5/%126-$$ [Z¬ê N\f! Ïäj.êST UVWXÄÚYsZ[Ø6eJ  f X! STUVØ6eJ 83 KŠ # !` $` STUV IR99S sZ[ Qq ŒG Lq ŒeJ KŠ ! µ$[6 3?> ê»ˆ ! 2“ Qq3>- G Lq3>- çÛ÷#ËKŠ !Qq3>- KŠ0Œƒf Q ] YMM PV Öè R Ùó—[Ùd ! -¥d ! ±G Ÿ  ] 7&$02 _6i K@=&21G [ 99 sZZ ¬m `Xf! hÝiØ6KŠ[eJèkš) °  f Q! STUV Qq3>-83 KŠ !!Lq3>- KŠ0Œƒf _! äÌè|Ù.w[‚ d ! §8©ê R NMM PV! &e0ŒÍ 7&$02 }C ! i K@=&21G [ 99 sZZ¬m· `Xf! i R@%126-B $$ 99 sZZ¬m NQf 2“ T?>=>? ›sCSTUV IR99 R sZ Q ™eJ#ˌw !?7; C›™èœž ! sZO z X \NR PV! ÐWX NM`sB !Lq JWX) $Lq< ,A3% è NR PV!Qq$Qq<,A3% è X\Y PV! ] Qq ŸŒè V/&B> Ønf  f _! STUV Lq3>-83 KŠ # !` %` STUV IR99R sZ`sBZ¬}~ ! STUVêHA° ! ýøUVHÉÌ ! m  ?7; ` sBZ¬eJ#Ë}C ! >Cª ¡‰H6  € TSTUVWXÄÚYsZIR99R%
iýøÉÌ
K@=&21G! R@%126-ÑHA°ÉÌ¢tÂÎê R ª ! ]i8 # 6| # À£ # a¤ # ÒW # ¶GÎê|§ ª ! j.STUViýøÉÌ K@=&21G! R@%126-B
 ¡‰H/–
!
`sBeJZ¬å]
`Mf

_
!
iHA°ý[b£øÉÌ[
11
sZZ¬Œ]
NMf
_
!
ƒf
L
f
L!
STUViª¦ÉÌWXÄÚY
sZ`sBZ¬Hå—
# #`
STUVWXÄÚYsZ
IR99R
[È+¼m

XSTUV
IR99R
sZ[OzÚÿ
8->1c-LQN-
{
QY
ï{
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!>V%&! XMRR
ôwM^<[
!
“
F1!
ÑC:1Cª#ËYy
!
Y
yäÌÍx€Xq‡/_R&
!
ƒf
Y!
y™¾[t
™è
X

!R
]
Q NMM PV
c^
!
êÚÿ
8->1c”
R
]
X _MM PV
c^
!
ê
IR99R
Oz

f
Y!8->1c<11
[»{Yy
# $`
¥Äçv]§#^’
7CXR
r[±²¼m
XYy[
IR99R
OzäÌÊJ
!
i{Yy¾[
8-AÚÿôw
!
1ÜÈ+¼mÚÿ
8-AIR99S
Í
83
Ïä§Æ¾Ø&e
!
Xž»/¾
XªM^·
7CXR
r±²¼m

*ó—[¨·©
ê
`X oRM
Q
[çv/_Ùd
!
i-&[çvjC6
A[·§8DÄ
!
ƒf
Zf
!
*Ïä)êST
UV
IR99R
sZ*]§#^’r»/¼m

R:7CXR
Y±²
&X:7CXR
±²
&Q:8-AY±²
&_:8-A±²
&L:8-AY±²
&Y:8-A±²

f
\!1T1<8>@-
q‡/_
‡, STUV‘XêeÚ6 ! ‚& €X # Ò ¦X # ŒX # X‡‘¢HXÙÚ ! Œ WX6Lä ãѧ㠠WXêÉÌÿ~ߨ[‚&Ì[Ñ©Š Ì[ ! WX[ÄÚN8iߨCÉÌãz€ÅGÕ 6^Ä̊bàÉ[ãÌä·EFÓ§ ! WXÄÚ YJ[yWXôÏ[‰ŠYg| ! êSTUV[‘ XÄÚµqhÿ ! Zf ! WXÄÚYe[Àê›w EFkSTUV‘X[\·GãÌä·  STUVWXÄÚYsZWX)Ozê X _X_ PV! WX NM`sB ! çvjC6A[·ê `XgQ oRM Q ! iýøÉÌ[`sBeJZ¬Àm `Mf! ib£øÉÌ[`sBZ¬b] NMf _ ! s Z~A‡  ì£7?†_STUV IR99R sZ [OeJ>#Ë_¼m6 ! CsZ[ài # ÔI G]XôÏ~*r[E“4Ü>‚™NE
!
Z
fª¾Yè•#|äò҃„

‘’“”%5)-!C(&/%C!#%P/*9-:A#(P/<0B)2#(00/604G%/0( 9(:7/#(I! R`LL!XR_,R_`:  _ (!C3! d#)*+7! .4).!(2&:&/))*! G#G2(%a<2/))5 2004(0V(G6G(KV%(00/) /604G0(*()(6%/I
2%/VG&(VV#B2G>=/%G#5 K1Q(=&21G9(:8&)28#B0/&!
XMM_! RYR,N\:
L (!b() ++!d#)be!J()ce!(2&:&/))*)5 G#%G2(2/) /604G0(<()G/5)**()(6%/II40WI(&/)9(:
=/&7/3(V! XMRM! Q\, Y`L:
Y (!./4a)(A!ABI/F0WC!AWE/%)>!(2&:#G2(%a2/)/62F/I(IP(,-61J-!:2()(6I&B()G/5)*
6/%04G%/0(0B)2#0(9(:@()(!RRXQ: (!>WHD!AWB=!Da4#W/D! (2&:>)&B00/604G%/0(0B)<
2#0(()(0)G2)5 V#B&/*()(2G%(<
&2/)0#V09(:-KV 7/2! XMMX! LQ,YR:  N (!=(G%2BT3! 1#F9!.))# C:A#(G&/))**()(2GIV<
V)*)5(KV0(&/G40/6
Ia(9(:82&>G5 1G,1>!R`NY!NN,`M``:  ` (!C)*&(1 -! TB(%9=:&/))*)5 (KV%(00/) /604G%/0(0B)<
2#$0(6%/I04*)(9(:8&)28#B0/&! XMMR!RLN, RX`: RM(!U4*&I=b! .$%)*2/) @?! 740# T3:14G%/0(2%)0G&%(*4&2/)/604GG2E2B) 2#(*:21B
&/9(:8%/G?2&>G5 1G,1>!XMMX!``,RMN\Y:
RR(!
ÇSK
!
 ¨«
!
( Û
!
‡
:
WXCSTUVÈ7Ìãzi
‘XW³[EF
9(:
¿Àðø_
!XMM\!QLRQ)*1 A!C4/98:-)#)G(I()2/6)5 V/&B0G< G#%5(V%/54G2/) ) 040V()0/) G4&24%(0/6V%/2/G/%)<&W(P/5(0
-YQN-
{
QY
ï{
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!>V%&! XMRR
6%/II&6(1,-5/:51:&&PB2#(552/) /6V42%(0G)(9(: 7/2(G#)/&C(2! XMM\! X`,_`L:
>B1ATI1/GT1BC0CV /CHAPLGAKK0BC/C/1FK0KBMKITGBKA
KFCDE/KAVACAMGB.2+034,55(+/
=-?@.()*&)*
R
! T,>?#()*&
R!X
! +;>OJ()*#4
R!X
!
! c>?@1#()*G#/ R ! d#c)*#/)*
Q
! b-?@4/0/)*
R
RgO!-!5&130:-&&K&6-)2K!&(-2! >5;2<-8&(-!=T>5(18)2P116 ;%(-)52!5(2()!-)&0&!&(13
0:-&&K&6-)2K!&(-2!M5/& Xg+)52!=13+1(&!(=6 H-1!&):121%=! .:&L-6 +1(&!(=
<-8&(-!=! E-?QRRQMM!0:& Q >5:1!-% 01@! E!6@! *-, YYRXMM! 0:-0(/6I&6(-,-5/13-)-2&)5 (KV%(00/) )&B00! 2/V%/E5(2#(2#(/%BP006/%G# 2#(%(&2/)0#V P(2F(() V/&B0GG#2&)504G0(G2E2B:>ADEBH,>GG/0(*()(/) @()7)W!VV&G2/) 2#(2(G#)/&/*B/63A<83)5 3>-! G&/)(2#(64&&()*2# /6
I@13-)-2&@A%*(2*()(IV&6(5 F2# AE(G2/%F02%)06/)&B00::AKI1D, >64&&()*2# GT?>()G/5)*04G%/0(0B)2#0(F00/&2(5 6%/I2#(I@13-)-2&! )I(5 IR99S! 2#(@()<
7)WGG(00/) )4IP(%0.eNLYNQL!2#(GT?>0X \NR PV ) &()*2# G/)2))) /V() %(5)6I(/6X _X_ PV ()G/5)*NM\ I<)/G50F2# V%I00/6`XgQ oRM Q !2#(5(54G(5I)/G50(4()G(/6I@13)2&04G%/0(0B)2#0(0#%(0`Lf
5()22BF2# K1Q( =&21G>JLQMLYN%(0`Mf 5()22BF2# R)-6-5/%126-$$$>JLQMLY\%& 0#$%(0I/%(2#$) NMfF2# /2#(%
I/)/G/2B&(5/)/40V&$)20:2BCT1IK0BC, &/)(5 2#(04G%/0(0B)2#$0(*()($)5 )54G(5 $) /PE/40P$)5 04GG(0064&B:
aAF WBGHK(!I&6(1,-5/13-)-$2&& 04G%/0(0B)2#$0(& *()(G&/))*& (KV%(00/) $)$&B00
HB0,!+b%#)QT[T..#+!!+(+%
$
•–$Q
!
—˜™
%
œH;žD
£©Ñ™m]pßÞài
!
EF#$%£©º»
FFFgGHGIIgG/IgG).
EFrá
/!
-â]pãa
!

¶·º_®Ë
$
ޙmº®ài[°â*¶·n“öãa
%
ѱ4ãaö
X
¢ãa…š
!
ãaÚà¾Hå
æw½ç[èféê

ë¾ç*ì)síîÑW¸ØUsmï;ðñj\[“
!
üB
!
€¼
‡

üèò#ó½ôõW¸
,RQNRMR\NNYR!XRQRMQNL]^^gG/I
-\QN-
{
QY
ï{
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!>V%&! XMRR

  鄂ICP备06018747号 Copyright @ 2017
  植物通 All Rights Reserved
  Email:23198511@qq.com
回顶部
51La