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作 者 ：魏元锋,林晓,张宁,冯怡

期 刊 ：中国中药杂志 2011年 36卷 9期 页码：1245.

关键词：大川芎方；效应组分；UPLC-MS；移血成分；

Keywords:DCXF, active parts, UPLC-MS, constituents absorbed into blood,

摘 要 ：目的: 研究硝酸甘油致实验性偏头痛模型大鼠灌胃大川芎方效应组分后血中移行成分及其归属。 方法 :以UPLC-MS作为分析手段,以大川芎方效应组分、川芎效应组分、天麻效应组分、天麻素、阿魏酸、洋川芎内酯I作为对照,综合分析总离子流色谱图、质量色谱图及质谱图等信息,通过对比各色谱峰的保留时间及质谱图特征,确认硝酸甘油致实验性偏头痛模型大鼠灌胃大川芎方效应组分后血中移行成分及其归属。 结果 :大川芎方效应组分给药后血浆中共发现10个入血成分,其中4个为天麻素等天麻效应组分中原型成分,6个为阿魏酸、洋川芎内酯I等川芎效应组分中原型成分。 结论 :本研究将为阐明大川芎方效应组分药效物质基础提供依据。

Abstract:Objective : To study the constituents absorbed into blood following the intragstric administration of Dachuanxiong Fang active parts(DCXF) to the experimental migrainous model rats induced by nitroglycerin. Method : The UPLC-MS was used as the analytic method. The DCXF, Chuanxiong active parts, Tianma active parts, gastrodin, ferulic acid and senkyunolide I were used as the control samples. The information on the total ion chromatogram, mass chromatogram and the mass spectrogram were synthetically analyzed to confirm the constituents absorbed into blood. Result : Ten of the DCXF constituents were detected in the rats plasma post the intragastric administration of DCXF , in whitch four including gastrodin came from Tianma active parts and six including ferulic acid and senkyunolide I from Chuanxiong active parts. Conclusion : The findings abtained from the study can provide the useful information for the determination of bioactive substances of the DCXF.

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