Abstract:Objective: To observe effect of astragaloside IV on gene expression profile of rat myocardium.Method: Astragalosid IV 5 mg·kg-1 ·d-1 was administrated intraperitoneally to male Wistar rats for 2 weeks and control rats were given same volume of solvent for astragaloside IV.Left ventricular myocardium following astragaloside IV treatment and the same regions of control group were used for gene microarray analysis.The cDNA microarray was scanned for the fluorescent signals.Differential expression genes and gene function were analyzed using related software respectively.Result: Seventy two genes were up-regulated by astragaloside IV significantly.Among them,biological characteristics of 39 genes were definite.After analysis on their function,the results showed that the effect of astragaloside IV on blood vessel development genes was most markedly up-regulated and its enhancement on genes related with cardiac muscle development,cell skeletal protein expression and cell contraction were also significant.Conclusion: Astragaloside IV could improve genes related to development of blood vessel and myocardial contraction in rat left ventricle.