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作 者 ：谢炜,于云红,赵云燕

期 刊 ：中国中药杂志 2010年 35卷 12期 页码：1619.

关键词：柴胡疏肝汤；戊四氮；癫痫；荧光脱氧葡萄糖；谷氨酸；天门冬氨酸；

Keywords:Chaihu Shugan Tang, pentylenetetrazole, epilepsy, 2-NBDG, glutamate, aspartate,

摘 要 ：目的: 研究中药复方柴胡疏肝汤对戊四痰(PTZ)慢性点燃大鼠皮层、海马(不同脑区)兴奋性的影响。 方法 :以PTZ腹腔注射建立大鼠慢性点燃癫痫模型,选取完全点燃大鼠随机分为模型组和治疗组,分别给予生理盐水、丙戊酸钠和柴胡疏肝汤高、中、低剂量连续灌胃治疗4周。分别应用荧光成像技术及HPLC检测大鼠不同脑区6- -2-脱氧葡萄糖(2-NBDG)的含量及谷氨酸(Glu)、天门冬氨酸(Asp)的浓度。 结果 :柴胡疏肝汤高、中、低剂量组均等显著降低癫痫大鼠不同脑区内的2-NBDG含量及Glu, Asp浓度(P<0.01)。 结论 :柴胡疏肝汤能降低PTZ慢性点燃大鼠不同脑区内葡萄糖的代谢,通过降低不同脑区内兴奋性神经递质的含量,可能是其降低大鼠不同脑区兴奋性的机制之一。

Abstract:Objective : To study the effect of the Chinese compound prescription Chaihu Shugan Tang (CHSGT) on the excitability in the cerebral cortex and hippocampus (different brain regions) of pentetrazole (PTZ)-kindled chronic epileptic rats. Method : To establish the model of chronic kindling rats intraperitoneal injected with pentylenetet. Fully kindled rats were randomized into control and experimental groups for intragastric administration of normal saline (control, model), Sodium Valproate and CHSGT at the high, medium and low doses for 4 consecutive weeks. The content of 2-NBDG, the glutamate (Glu) and the aspartate (Asp) in different brain regions of rats were detected by fluorescence imaging techniques and HPLC assay respectively. Result : CHSGT at the high, medium and low doses all significantly decreased the content of 2-NBDG, the Glu and the Asp in different brain regions of chronic epileptic rats (P<0.01). Conclusion : CHSGT can inhibit the excitability in different brain regions of PTZ-induced epileptic rats, by decreasing the level of excitatory neurotransmitter maybe one of its antiepileptic mechanisms.

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