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作 者 ：赵雯红,陈志强,张江华,孙玉凤,王月华,王会青

期 刊 ：中国中药杂志 2010年 35卷 6期 页码：768.

关键词：益气养阴消癥通络中药；糖尿病肾病大鼠；p38MAPK信号通路；

Keywords:supplementing Qi-nourishing Yin-dispersing blood stasis-dredging collateral herbs, DN rats, p38MAPK signaling pathway,

摘 要 ：目的: 探讨益气养阴消癥通络中药对早期糖尿病大鼠肾组织p38MAPK信号通路的作用。 方法 :将SD大鼠随机分为6组:单纯肾切组、模型组、厄贝沙坦组、中药低、中、高剂量组,按各组相应药物剂量灌胃。于第6周末检测各组大鼠24 h尿蛋白(UPro)量及肾功能水平;肾皮质p38MAPK mRNA,p-p38MAPK蛋白表达水平。 结果 :厄贝沙坦、中药低、中剂量组与模型组比较Upro量显著下降(P<0.05);治疗组与模型组比较,肾功能明显改善(P<0.05),且p38MAPK mRNA,p-p38MAPK蛋白表达水平显著下降(P<0.05)。 结论 :益气养阴消癥通络中药可能通过抑制p38MAPK 信号通路表达,发挥对糖尿病肾病大鼠的治疗作用。

Abstract:Objective : To study the effect of supplementing Qi-nourishing Yin and dispersing blood stasis-dredging collateral herbs on p38 mitogen activated protein kinase (p38 MAPK) signaling pathway in the kidney of early diabetic rats. Method : Dividing SD rats randomly into 6 groups: Simple nephrectomy group, model group, irbesatan group, traditional Chinese medicine (TCM) low dose group, TCM middle dose group and TCM high dose group. Each group of rats was fed with the corresponding dose of medicine. After 6 weeks, detecting 24 h urine protein (UPro) level, renal function, p38 MAPK mRNA and p-p38MAPK protein level. Result : UPro levels of irbesatan group, TCM low group and TCM middle dose group decreased significantly (P<0.05), compared with that of the model group. Renal function of the treated groups was improved greatly and their p38 MAPK mRNA and p-p38MAPK protein levels decreased significantly (P<0.05), compared with those of the model group. Conclusion : Supplementing Qi-nourishing Yin-dispersing blood stasis-dredging collateral herbs could treat DN rats effectively by inhibiting the expression of p38 MAPK signaling pathway.

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