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期 刊 ：中国中药杂志 2006年 31卷 03期 页码：236.

关键词：麻黄；地龙；平喘；平滑肌张力；短路电流；

Keywords:Ephedra sinica, Ephedrae & Pheretima, asthma, tensino, short-circuit current,

摘 要 ：目的:考察药对麻黄、地龙用量配比及可能的平喘作用机制。方法:观察麻黄、地龙3种用量配比(1∶1,1∶3,1∶9)水煎液对10μmol.L-1卡巴胆碱(carbachol,CCh),10μmol.L^-1组胺(histamine,His)引起离体气管平滑肌张力增加的松弛作用,以及对10μmol.L^-1CCh引起的离体气管上皮短路电流增加的抑制作用。结果:对于10μmol.L^-1CCh引起的离体大鼠气管平滑肌收缩,麻黄地龙3种配比水提物的舒张作用随剂量增加而增强,1∶1,1∶3水提物的IC50分别为7.5,15 mg.mL^-1,1∶9水提物不能产生对CCh引发最大收缩抑制50%的效应;对于10μmol.L^-1His所引起的离体豚鼠气管平滑肌收缩,麻黄地龙3种配比水提物的舒张作用随剂量增加而增强,1∶9,1∶3,1∶1水提物的IC50分别为0.19,0.61,1.8 mg.mL-1;在抑制CCh引起的离体大鼠气管上皮短路电流增加方面,三者的作用强度顺序为1∶3>1∶1>1∶9,三者没有显著性差异。结论:麻黄、地龙药对具有舒张离体气管平滑肌和抑制气管上皮离子分泌的作用,其作用机制包括抗CCh和抗His作用。1∶3是麻黄、地龙药对中的最适配比。

Abstract:Objective: To study the proportion and mechanism of relieving asthma of drug partnership comprising herbal Ephedrae & Pheretima.Method: To study relaxant effect on 10 μmol·L^-1 carbachol(CCh) and 10 μmol·L^-1 histamine(His) precontracted isolated tracheal rings and lowering effect on short-circuit current(Isc) increase induced by 10 μmol·L^-1 CCh with 3 proportions of 1∶1,1∶3,1∶9 extract.Result: 1∶3 proportions dose-dependently relaxed CCh-precontracted isolated tracheal rings,I₅₀ of 1∶1,1∶3 is 7.5,15 mg·mL^-1 respectively,1∶9 could not produce 50% inhibition effect on CCh-evoked contraction;3 proportions also dose-dependently relaxed His-precontracted isolated tracheal rings,IC_(50) of 1∶9,1∶3 and 1∶1 is 0.19,0.61,1.8 mg·mL^-1 respectively.On the other hand,the orders potency of the decrease effect on CCh-evoked short circuit current increase is 1∶3 >1∶1> 1∶9.The difference is not significant(P<0.05). Conclusion: Herbal Ephedrae & Pheretima had tracheal muscle relaxant and epithelium ion secretion inhibition effect,its mechanism of relieving asthma involved anti-CCh and anti-His effect 1∶3 was the most appropriate dosage ratio in the anti-asthmatic drug partnership.

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