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期 刊 ：中国中药杂志 2005年 30卷 12期 页码：930.

关键词：脑微血管内皮细胞；大鼠；缺血再灌注；牛胆酸；

Keywords:Cerebral microvascular endothelial cell, rat, ischemia-reperfusion, cholic acid,

摘 要 ：目的:建立脑微血管内皮细胞体外缺血再灌注模型,并观察了牛胆酸对其影响。方法:体外培养大鼠脑微血管内皮细胞,以氧糖剥夺(Krebs液)再复氧复糖模型模拟缺血再灌注损伤,并用牛胆酸进行了干预,用MTT比色法测定A值代表细胞生存活性。结果:氧糖剥夺4h再复氧复糖12h可造成培养大鼠脑微血管内皮细胞损伤,牛胆酸干预后A值明显高于模型组(P<0.01)。结论:牛胆酸可显著减轻脑微血管内皮细胞体外模拟缺血再灌注细胞损伤。

Abstract:Objective: To establish an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells of rats and observe the protective effect of cholic acid. Method: Cultured rat microvascular endothelial cells were subjected to the oxygen-glucose deprivation (OGD) (Krebs solution) and recovery of oxygen-glucose, which simulated in vitro ischemia and reperfusion injury,and treated with cholic acid. The A value was measured with MTT chromatometry. Result: Cultured cells were impaired after OGD for 4 hours and recovery of oxygen-glucose for 12 hours, the A value of the cells treated with cholic acid was significantly higher than that of the cells without treatment(P<0.01). Conclusion: Cholic acid could obviously protect rat cerebral microvascular endothelial cells from injury induced by an in vitro ischemia-reperfusion.

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