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期 刊 ：中国中药杂志 2005年 30卷 23期 页码：1866.

关键词：桃红四物汤Ⅱ号；B16黑色素瘤；VEGF；KDR/FLK-1；血管生成；

Keywords:Taohong Siwu decoctionⅡ, B16 Melanoma, VEGF, KDR/FLK-1, agiogenisis,

摘 要 ：目的:观察桃红四物汤Ⅱ号对小鼠B16黑色素瘤生长、血管内皮生长因子(VEGF)和血管内皮生长因子受体-2(KDR/FLK-1)表达及肿瘤微血管密度(MVD)值的影响。方法:采用C57BL/6J小鼠皮下接种B16黑色素瘤细胞建立模型,分别给予2.5,5,10 g.kg^-1桃红四物汤Ⅱ号水煎剂、环磷酰胺0.05 g.kg^-1及联合用药桃红四物汤Ⅱ号10 g.kg^-1+环磷酰胺0.025 mg.kg^-1,检测各组肿瘤体积、重量、VEGF和KDR/FLK-1表达及肿瘤MVD值。结果:桃红四物汤Ⅱ号5,10 g.kg^-1及联合用药组肿瘤体积、重量、VEGF和KDR/FLK-1表达及肿瘤MVD值均较生理盐水组明显降低(P<0.01)。结论:桃红四物汤Ⅱ号能抑制小鼠B16黑色素瘤的生长,其抗肿瘤机制可能与抗血管生成作用有关。

Abstract:Objective: To investigate the effects of Taohong Siwu decoctionⅡon B16 melanoma in mice and the underlying mechanism.Method: C57BL/6J mice bearing B16 melanoma were used in this study.The experimental groups were treated respectively with Taohong Siwu decoctionⅡ at doses of 2.5,5 and 10 g·kg^-1 and cyclophosphamide at 0.05 g·kg^-1,Taohong Siwu decoctionⅡat 10 g·kg^-1 plus cyclophosphamide at 0.025 mg·kg^-1.The tumor volume and weight,the expression of VEGF and KDR/FLK-1 and the amount of MVD were measured.Result: The tumor volume and weight,the expression of VEGF and KDR/FLK-1 and the amount of MVD were reduced significantly after treatment with Taohong Siwu decoctionⅡat the doses of 5 and 10 g·kg^-1 and Taohong Siwu decoctionⅡcombined with cyclophosphamide as compared with normal saline-treated group(P<0.01).Conclusion: Taohong Siwu decoctionⅡcan inhibit the growth of B16 melanoma in mice and attenuate the expressions of VEGF and KDR/FLK-1,suggesting that Taohong Siwu decoctionⅡ produces the antitumor effect via a possible antiagiogenisis mechanism.

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