Abstract:Four compounds were isolated from the roots of Euphorbia nematocypha. Three of them were identified as A‘-neogammacer-22 (29)-en-3β-ol(18β, 21α) (Ⅱ) ,jolkinolide E(Ⅲ) and β-sitosterol (Ⅳ). Among them , Ⅱ was obtainedfrom the genus Euphorbia and Ⅲ from this plant for the first time.