Abstract:A method of thin layer chromatographic separation and ultraviolet spectrophotometric determination of tangshenoside Ⅰ in Codonopsis pilosula is described. A comparison of the contents in various samples is made. The contents of tangshenoside Ⅰ in frosted sample have been found to be twice as much as in normal drug. The recovery of tangshenoside Ⅰ is 99.92% and the coefficient of variation of eight samplings is 1.77%.