Abstract: Objective To clone 3-dehydroquinate synthase cDNA from Echinacea angustifolia and in-vestigate its tissue expression characteristics in various tissues. Methods By using homology cloning andRACE-PCR，the cDNA encoding 3-dehydroquinate synthase was amplified with cDNA library of culturedplantlets as the template. The specificity expression profile in different tissues including roots，stems，leaves，and flowers of E. aungustifolia was investigated by semi-quantitative RT-PCR as well. Results The full length cDNA of 3-dehydroquinate synthase (named as EanaroB) had 1 424 by with an open read-ing frame encoding 442 amino acids of protein and its EanaroB was around 80%homologous with the se-quences of 3-dehydroquinate synthase from other plants. The sequence was reported to the GeneBank andcoded as EU293857. The results of semi-quantitative RT-PCR indicated that the expression of EanaroBwas detected in different tissues，while only the expression in leaves and flowers reached to a high level. Conclusion The cDNA encoding EU293857 from E. angusti folia is cloned and reported. This work un-derlays the first step for exploring the pathway of caffeic acid derivatives biosynthesis and improving thecontent of caffeic acid derivatives including phenylethanoid glycosides.