Abstract:The vitrification of Daphne plantlets in vitro was affected by the size of the tip explant and the concentrations of cytokinin in the media, much more vitreous plantlets were induced when smaller shoot tip explant and higher concentrations of cytokinin were used. The zonal structure of shoot apex of
vitreous plantlets appeared different from normal potted plants and normal plantlets in mainly aberration occurring in corpus and tunica.