Abstract:Field experiments with different maturity cotton cultivars and sowing dates were conducted at different sites to quantitatively study the effects of c ultivar characteristics, weather conditions (air temperature and solar radiation ), and crop management variable (N application rate) on the cotton boll maturation period and cottonseed biomass accumulation. The cotton boll maturation period was simulated by using the scale of physiological development time. Based on t he hypothesis of sink-determined, the cottonseed biomass accumulation model was then developed. The subtending leaf N concentration of cotton boll was simulate d with a semi-empirical equation, and used as the direct indicator of the N nut rition effect on cottonseed growth and development. The model was tested by inde pendent field data obtained in the Yellow River Valley (Xuzhou and Anyang) and t he lower reaches of Yangtze River Valley (Huaian) in 2005. The simulated values of boll maturation period showed reasonable agreement with observed values, with a root mean square error (RMSE) of 225 days for cultivar DSC-1, of 261 day s for cultivar KC-1, and of 275 days for cultivar AC-33B. The RMSE of cotton seed dry mass prediction was 95 mg·seed-1 for KC-1 and 82 mg·seed -1 for AC-33B, indicating that the model had a good prediction precision.