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作 者 ：王梅¹;高志奎¹;黄瑞虹¹;王惠英¹;张文丽¹;高荣孚²

期 刊 ：应用生态学报 2007年 18卷 01期 页码：63-68

关键词：茄；

摘 要 ：以耐热性较弱的黑贝一号圆茄和耐热性较强的黑贝二号圆茄为试材，热胁迫处理后采用植物效率仪PEA进行快速叶绿素荧光诱导曲线及其参数测定.结果表明：当温度高于40 ℃，PSⅡ结构受热胁迫影响较为敏感，表现为初始荧光F_o缓慢上升；PSⅡ原初光化学效率F_v/F_m和ΔF/F_m′大幅度下降，且黑贝二号F_v/F_m的半衰时间T₅₀和ΔF/F_m′的半衰温度t₅₀分别大于黑贝一号.较高的热胁迫剂量（48℃处理5 min或44℃处理20～30min）下，快速荧光诱导动力学曲线呈现OKJIP型，在700μs处出现与放氧复合体失活有关的K相.黑贝一号在44 ℃下处理20 min才有K相出现，黑贝二号则晚10 min出现.与35℃相比，在48℃，特别是在52℃的较高剂量热胁迫下，Strasser能量流动模型参数中的DI_o/RC有大幅度地增加，体现了热耗散对PSⅡ的较强保护能力.随着热胁迫温度的升高和热胁迫时间的延长，两品种的无活性中心F_vi/Fv显著增加.

Abstract:With lower-and higher heat-resistant varieties of eggplant (Solanum melongena L.) HeibeiⅠ
and Heibei Ⅱ as test materials, and by using Plant Efficiency Analyzer (PEA) from Hansatech,
this paper measured the fast chlorophyll a fluorescence transient and its parameters. The
results showed that PS Ⅱ construction became more sensitive to heat stress when ambient temperature
was higher than 40 ℃. The F_o went up slowly, and F_v/F_m and ΔF/F_m′came down dramatically.
Heibei Ⅱ had a longer semi-attenuation temperature of F_v/F_m (T₅₀) and ΔF/F_m′(t₅₀) than Heibei
Ⅰ. Under strong heat stress (5 min at 48 ℃ or 20-30 min at 44 ℃), the K-step in relation to
the inactivation of oxygen-evolving complex appeared in fluorescence rise at about 700 μs, and the
regular O-J-I-P transient was transformed to O-K-J-I-P one. The K-phase of HeibeiⅠand Heibei Ⅱ
appeared when the treatment time was up to 20 and 30 minutes at 44 ℃, respectively. In comparing
with 35 ℃heat treatment, the DI_o/RC in the parameters of Strasser′s specific energy fluxes model
was increased by a great extent under 48 ℃ or more heat stress, reflecting a strong safeguard of
energy dissipation to PS Ⅱ. When the temperature of heat stress increased from 35 ℃ to 52 ℃, the F_vi/F_v
of PS Ⅱ silent reaction centers of HeibeiⅠand Heibei Ⅱ increased remarkably.

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