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作 者 ：王长永^1,2;刘燕²;周骏¹;陈建群¹;钦佩¹

期 刊 ：应用生态学报 2007年 18卷 04期 页码：801-806

关键词：棉花；

摘 要 ：采用花粉粒染色法对转Bt基因棉的花粉漂移距离和强度进行了观测,并应用PCR法检测转Bt基因棉的基因流频率.花粉粒染色法监测结果表明：同株异花间的花粉散布频率显著高于异株异花间(P<0.01);靠近转Bt基因棉花粉染色区1 m处的平均花粉散布频率,在东、南、西、北4个方向分别为44.8%、48.9%、57.1%和21.5%,但随着距转基因棉田距离的增大,4个方向的平均花粉散布频率都呈下降趋势.PCR结果的统计分析表明,在25m内,花粉散布距离和方向对基因流频率有极显著影响(P<0.01),随着距转基因棉田距离的增大,基因流频率呈下降趋势,最远距离为25 m时的最高基因流频率为2.0%.

Abstract:By the methods of pollen grain dyeing and PCR analysis, this paper measured the distance and intensity of pollen dispersal, and determined the frequency of gene flow (FGF) of transgenic Bt cotton. The results showed that the frequency of pollen dispersal (FPD) among flowers was significantly higher on the same cotton plant than on different ones (P<0.01). The average FPD at a distance of 1 m from the dyed Bt cotton plot to its east, south, west and north was estimated to be 44.8%， 48.9%， 57.1% and 21.5%,respectively, and declined with increasing distance in the four directions. Within the range of 25 m, the distance and direction of pollen dispersal could affect FGF significantly (P<0.01), and FGF generally tended to decrease with increasing distance from the Bt cotton plot, with a frequency of 2.0% at the farthest distance of 25m.

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