全 文 ：菌 物 系 统 22（4）：666~668, 2003
Mycosystema
PESTALOTIOPSIS KARSTENII, A NEW RECORD OF ENDOPHYTIC
FUNGI FROM CAMELLIA SASANQUA IN CHINA*
Wei Ji-Guang1, 2 Xu Tong1, 3**
(1Agricultural & Biotechnical College, Zhejiang University, Hangzhou, 310029; 2Forestry College, Guangxi
University, Nanning, 530001; 3Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese
Academy of Sciences, Beijing, 100080)
中国茶梅内生真菌一新记录种*
韦继光 1，2 徐 同 1，3**
(1浙江大学农业与生物技术学院 杭州 310029; 2广西大学林学院 南宁 530001; 3中国科学院微生物研究所真菌地
衣系统学重点实验室 北京 100080)
中图分类号：Q939.5 文献标识码：A 文章编号：1007-3515（2003）04-0666-0668
In survey of diversity of endophytic Pestalotiopsis associated with Theaceae in Nanning, Guangxi
Province and Kunming, Yunnan Province, Pestalotiopsis karstenii, a dominant endophytic fungus
isolated from stem of Camellia sasanqua, is a new record in China.
Pestalotiopsis karstenii (Saccardo & P. Sydow) Steyaert, Bull. Jard. Bot. Etat. Bruxelles 19 :
305, 1949. Fig. 1
ºPestalotia inquinans P. Karsten, Hedwigia 30: 301, 1891, [non Pestalotia inquinans Cooke &
Harkness (1884)], fide Steyaert, ibid: 305, 1949.
ºPestalotia karstenii Saccardo & P. Sydow in Saccardo, Syll. Fung. 14:1030, 1899.
ºMonochaetia karstenii (Saccardo & P. Sydow) Sutton, Can. J. Bot. 47: 2091, 1969.
= ?Coryneum camelliae Massee apud Cooke, Grevillea 20:8, 1891.
=Monochaetia camelliae Miles, Mycologia 18: 167, 1926.
Colony on PDA white, effuse, margin nearly round. Mycelia superficial. Acervuli develop in
hyphae and give rise to conspicuous black spore masses. Conidiogenous cells discrete, ampulliform,
lageniform or subcylindrical, colourless, smooth, 7~16×2.5~3.2µm (mean 11×2.7µm) with up to 3
proliferations. Conidia fusiform, 4-septate, 15.6~31.2×4.6~6.1µm (mean 19.1×5.5µm), bearing
appendages; intermediate cells 3, subcylindrical to doliiform, pale brown and concolourous, wall
thick, smooth and slightly collapsed between the septa, together 11~20.2µm (mean 19.5µm) long
[second cell from the base 3.9~7.8µm (mean 4.4mm); third cell 3.3~5.9µm (mean 4.7µm) and fourth cell
3.9~6.5µm (mean 4.9µm)]; apical cell conic, colourless, thin-walled, smooth, 2.6~5.2µm (mean 4.1µm)
long; apical appendages tubular, coarse, flexuous, single, unbranched or branched irregularly along
their length resulting in 2 branches (rarely 3 branches); appendages or branches 5.4~28.2µm (mean

* Supported by the National Natural Science Foundation of China (国家自然科学基金项目 No.30270015)
** Author for correspondence: E-mail : xutong@zju.edu.cn
Received:2003-04-23, accepted: 2003-06-09
DOI:10.13346/j.mycosystema.2003.04.028
4期 韦继光等：中国茶梅内生真菌一新记录种 667

12.2mm) long; basal cell obconical with an obtuse base, smooth-walled, colourless, 2.5~6.5mm (mean
4.6mm) long; basal appendage absent; mean conidium length/width ratio= 3.47 : 1.
In stem of Camellia sasanqua Thunb., PSHI2002Endo402, 30 XI 2002, Nanning, Guangxi;
PSHI2002Endo671, 676, 684, 685, 686, 699, 11 XI 2002, Kunming, Yunnan. The living cultures were
deposited in the Agricultural & Biotechnical College of Zhejiang University.
Pestalotiopsis karstenii is somewhat similar to Pestalotiopsis manculans (Corda) Nag Raj (1985,
1988). Pestalotiopsis manculans was also isolated from leaf spots of Camellia japonica in Hangzhou
and healthy stem of the same host species in Hangzhou, Nanning and Kunming. It could be
distinguished from Pestalotiopsis karstenii by that the apical appendages are usually 2~3, occasionally
4~5, branched ; the apexes of the appendages are slightly spathulate; the median cells are versicoloured
and basal appendage is existent (Nag Raj, 1993; Ge et al., 1993).

Fig. 1 Conidia of Pestalotiopsis karstenii (bar=20mm)

One hundred and forty-seven Pestalotiopsis species have been recorded in China. These records
are due to the studies made by Tai (1979), Huang (1982), Sun & Ge (1990), Sun & Zhu (1990), Sun et
al. (1990), Zhu et al. (1991), Chen & Wei (1993,1997), Chen et al. (2002), Wei & Chen (1994), Qi
(1994, 2000), Zhao & Li (1995), Guo (2002), Wang et al. (2002) and Zhang et al. (2003).
Pestalotiopsis karstenii isolated from tissues of healthy stems of Camellia sasanqua as an endophyte
seems to be unrecorded from China previously.
The authors would like to thank Dr. L.D.Guo for pre-submission review of the manuscript.
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