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断穗小麦和节节麦黏壳、断穗基因SSR位点的遗传多样性



全 文 :书!#$%& 2015,35(8):10571066
JournalofTriticeaeCrops doi:10.7606/j.issn.10091041.2015.08.05
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:20150803
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:http://www.cnki.net/kcms/detail/61.1359.S.20150803.1523.005.html
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犵狌犾犪狋犪†‡K4ˆ‰ŠJ+,&†‡/e‹ghr狋犪狌狊犺犻犻†‡K‰ŠJ+,&cd、狋狔狆犻犮犪犾Œ‡K‰
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01234
:S512.9;S330    56789:A    5:;4:10091041(2015)08105710
犌犲狀犲狋犻犮犇犻狏犲狉狊犻狋狔狅犳犛犛犚犔狅犮犻犃狊狊狅犮犻犪狋犲犱狑犻狋犺犜犲狀犪犮犻狅狌狊犌犾狌犿犲犪狀犱
犅狉犻狋狋犾犲犚犪犮犺犻狊犌犲狀犲狊犻狀犎犲狓犪狆犾狅犻犱犠犺犲犪狋犪狀犱犜狉犻狋犻犮狌犿狋犪狌狊犮犺犻
犣犎犗犝犌狌狅狔犪狀,犔犗犖犌犠犲狀犼犻犲,犔犈犐犢狅狀犵狋犪狅,犆犃犐犙犻狀犵,犠犝犛犺犪狅狔狌狀
(BiotechnologyandGermplasmResourcesInstituteofYunnanAcademyofAgriculturalSciences,YunnanProvincial
KeyLabofAgriculturalBiotechnology,KeyLabofSouthwesternCropGeneResourcesandGermplasm
InnovationofMinistryofAgriculture,Kunming,Yunnan650223,China)
犃犫狊狋狉犪犮狋:ToinvestigatetheoriginoftenaciousglumeandbrittlerachisgenesinYunnanhuledwheat
(犜狉犻狋犻犮狌犿犪犲狊狋犻狏狌犿 ssp.狔狌狀狀犪狀犲狀狊犲),geneticdiversityandgeneticrelationshipofYunnanhuled
wheat,Tibetanwheat(犜.犪犲狊狋犻狏狌犿ssp.狋犻犫犲狋犪狀狌犿)and犜.狋犪狌狊犮犺犻犻werestudiedonsimplesequence
repeats(SSR)lociassociatedwithtenaciousglumeandbrittlerachisgenes,withmachawheat(犜.
犪犲狊狋犻狏狌犿ssp.犿犪犮犺犪)andspeltwheat(犜.犪犲狊狋犻狏狌犿ssp.狊狆犲犾狋犪)ascontrols.26pairsofSSRprimers
closelylinkedtotenaciousglumegenesTg2BSandTg2DSlocatedonthesecondhomologouschro
mosomesandbrittlerachisgenesBr3ASandBr3BSlocatedonthethirdhomologouschromosomes
wereinvolved.TheresultsshowedthatthegeneticdiversityofYunnanhuledwheatwasmoreabun
dantthanthatofTibetwheatatSSRlociofbothTg2BSandTg2DSandBr3ASandBr3BS.AtSSR
lociofTg2BSandTg2DS,therelationshipbetweenYunnanhuledwheatandTibetanwheatwas
moredistantthanthatbetweenTibetanwheatandmachawheat,andbetweenTibetanwheatandspelt
wheat.However,atSSRlociofBr3ASandBr3BS,therelationshipbetweenYunnanwheatandTi
betanwheatwascloserthanthatbetweenYunnanwheatandspeltwheat,andbetweenTibetanwheat
andspeltwheat.Butthoseresultscannotindicatethesourceoftenaciousglumeandbrittlerachis
genesinYunnanhuledwheat.Moreover,atSSRlociofTg2BS,therelationshipof狊狋狉犪狀犵狌犾犪狋犪sub
speciesin犜.狋犪狌狊犮犺犻犻and4subspeciesofhexaploidwheatwithbrittlerachiswasmoredistantthanre
lationshipof狋犪狌狊犺犻犻subspeciesin犜.狋犪狌狊犮犺犻犻and4subspeciesofhexaploidbrittlerachiswheatwith
brittlerachisandrelationshipof狋狔狆犻犮犪犾varietyof犜.狋犪狌狊犮犺犻犻and4subspeciesofhexaploidwheat
withbrittlerachis.ItwasshownthatdonorofDchromosomeofhexaploidwheatwithbrittlerachis
maynotbelongto狊狋狉犪狀犵狌犾犪狋犪subspecies,butmaybegenotypeof狋犪狌狊犺犻犻subspecies.
犓犲狔狑狅狉犱狊:Wheatwithbrittlerachis;犜狉犻狋犻犮狌犿狋犪狌狊犮犺犻犻;Genesofbrittlerachisandtenaciousglume;
SSRlocus;Geneticdiversity
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2 45167
2.1 PQ>?RHST!D犜犵UVWXA犛犛犚
YZ[A\]^_`
2.1.1 BKTg2BS-.PQRS/SSRAO/]^_`L
PTg2BSjkýþðñ…2¾SSRòó

Œ1)‹$Œ102n÷õê!CŸ )20
ä¾â“Uìjk

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ã¾â×â…90%(Œ2)。Œ56!”^
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9

åo`…Y†H_¼äi·!…æç{
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ä”^… 犎t=0.0187,U”^؅ 犎s =
0.0181,ãèU¿×…jkH_¼,x”^,…
jkH_¼ãègæÞéê

åoº4ä”^…
jkH_¼ãèëŠe,”^ØhUÅ}”^b
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Y†Ìöàk샄‡Œ¿”^Øh
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kµ ŒTg2BS ì < Ü … ï Ê ï ;犖m =
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a1 bcA犛犛犚2d7efghiUVWXA!D
犜犪犫犾犲1 犛犛犚犿犪狉犽犲狉狊狌狊犲犱犪狀犱狋犺犲犵犲狀犲犮犾狅狊犲犾狔犾犻狀犽犲犱
SSRòó
SSR
markers
ýþðñ…jk
Genesclosely
linked
‚öNW
Reference
SSRòó
SSR
markers
ýþðñ…jk
Genesclosely
linked
‚öNW
Reference
WMC25 Tg2BS [5] CFA2076 Br3AS http://wheat.pw.usda.gov/
WMC154 Tg2BS [5] GPW2016 Br3AS http://wheat.pw.usda.gov/
WMC112 Tg2DS [8] WMC169 Br3AS http://wheat.pw.usda.gov/
BARC168 Tg2DS [8] WMC777 Br3BS [6]
GWM261 Tg2DS http://wheat.pw.usda.gov/ GWM285 Br3BS http://wheat.pw.usda.gov/
WMC503 Tg2DS [20],http://wheat.pw.usda.gov/ CNL3D Br3BS http://wheat.pw.usda.gov/
GPW332 Tg2DS http://wheat.pw.usda.gov/ PSP3081 Br3BS http://wheat.pw.usda.gov/
GWM455 Tg2DS http://wheat.pw.usda.gov/ GWM131 Br3BS http://wheat.pw.usda.gov/
WMC25 Tg2DS http://wheat.pw.usda.gov/ WMC291 Br3BS http://wheat.pw.usda.gov/
WMC470 Tg2DS http://wheat.pw.usda.gov/ BARC164 Br3BS http://wheat.pw.usda.gov/
WMC087 Tg2DS http://wheat.pw.usda.gov/ BARC344 Br3BS http://wheat.pw.usda.gov/
GWM484 Tg2DS http://wheat.pw.usda.gov/ BARC218 Br3BS http://wheat.pw.usda.gov/
GWM2 Br3AS [6] GWM108 Br3BS http://wheat.pw.usda.gov/
GPW2118 Br3AS http://wheat.pw.usda.gov/
a2 jklmnopR犜犵2犅犛!D犛犛犚YZ[A\]qr
犜犪犫犾犲2 犌犲狀犲狋犻犮狏犪狉犻犪狋犻狅狀狅犳犺犲狓犪狆犾狅犻犱狑犺犲犪狋狑犻狋犺犫狉犻狋狋犾犲狉犪犮犺犻狊犪狋狋犺犲犛犛犚犾狅犮犻狅犳犜犵2犅犛犵犲狀犲
”^ Subspecies NI 犖a 犖e  犎  犐 NPL PPL/%
°±! 犜.犪犲狊狋犻狏狌犿ssp.犿犪犮犺犪 1 1.0000 1.0000 0 0 0 0
¥²˜{! 犜.犪犲狊狋犻狏狌犿ssp.狊狆犲犾狋犪 1 1.0000 1.0000 0 0 0 0
56! 犜.犪犲狊狋犻狏狌犿ssp.狔狌狀狀犪狀犲狀狊犲 61 1.7500 1.0567 0.0510 0.1090 15 75
i·! 犜.犪犲狊狋犻狏狌犿ssp.狋犻犫犲狋犪狀狌犿 39 1.4000 1.0228 0.0214 0.0498 8 40
“B Total 102 1.9000 1.0421 0.0393 0.0917 18 90
  NI:äêâ;犖a:Uìjkâ;犖e:’ÓUìjkâ;犎:Nei’sjkH_¼Ôâ;犐:ShaanonH_¼Ôâ;NPL:HÕ¼ì<â;PPL:H
Õ¼ì<Öïâ

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[1]DongYS(€‚).Rarespeciesin狋狉犻狋犻犮狌犿andtheirutiliza
tioninbreeding[J].犛犮犻犲狀狋犻犪犃犵狉犻犮狌犾狋狌狉犪犛犻狀犻犮犪(KFG8
%
),1979(3):17(inChinesewithEnglishabstract).
[2]PengJH,SunDF,NevoE.Wildemmerwheat,犜狉犻狋犻犮狌犿犱犻犮
狅犮犮狅犻犱犲狊,occupiesapivotalpositioninwheatdomestication
process[J].犃狌狊狋狉犪犾犻犪狀犑狅狌狉狀犪犾狅犳犆狉狅狆犛犮犻犲狀犮犲,2011,5(9):
11271143.
[3]PengJH,SunDF,NevoE.Domesticationevolution,genetics
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281301.
[4]FarisJD.Wheatdomestication:keytoagriculturalrevolutions
pastandfuture[M].TuberosaR,GranerA,FrisonE.Ge
nomicsofplantgeneticresources(Volume1.Managing,se
quencingandmininggeneticresources).Dordrecht:Springer
Press,2014:440460.
[5]FarisJD,ZhangZC,ChaoSM..Mapbasedanalysisofthe
tenaciousglumegeneTgB1ofwildemmeranditsrolein
wheatdomestication[J].犌犲狀犲,2014,542(2):198208.
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