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作 者 ：雷秀清, 李力, 黄建忠

期 刊 ：生物技术通报 2014年 0卷 5期 页码：45-51

关键词：次级代谢；核糖体工程；异源表达；放线菌；

Keywords:Secondary, Netabolites, Ribosome engineering, Heterologous expression, Actinomycetes,

摘 要 ：放线菌的次级代谢产物一直是抗生素等药物开发的重要来源。通过基因技术来提高放线菌次级代谢产物产量的方法已被广泛接受。结合近年的研究成果，概述了利用基因技术过表达或者敲除代谢途经关键基因及加倍基因簇，诱变核糖体相关蛋白的基因，以及异源表达外源基因簇等提高次级代谢产物产量的方法。旨在为寻找新药及改造高产菌株提供相应的技术参考。

Abstract:Actinomycetes secondary metabolites have been the important sources of developing antibiotics and other drugs. Gene technology has been widely practiced for improving the yield of Actinomycetes secondary metabolites. This review summarized a series of technical methods used for improving Actinomycetes secondary metabolites，consisting of over-expressing the key structural genes and/or positive regulation genes，knocking out the negative regulatory genes，doubling the clusters，mutating ribosomal protein genes，and expressing the foreign cluster heterologously. These methods could be used for new drug discovery and strains improvement.

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