全 文 ：A Quick New Method for Determining Light Response Curves of
Photosynthesis Under Field Light Conditions
JIANG Gao-Ming HE Wei-Ming
( C e n t e f o r P h E o d o g y a n d B i a d i v e r s i i y C m c ~ , I ~ ( ~ 4 B o t a n ~ , lkCChineAmdemyqfScienoes, Beijing100093)
Abstract A quick new method for determining light response curves of photosynthesis under field
light conditions has been developed. By using only a potable photosynthesis system ( U 4 , UK)
under full sunlight condition, we could obtain a series of assimilation rate-light (A-light) response
curves by varying the angle of incident light rays to the sensing head to get a series of light intensi-
ties (0-2 200 p n ~ l * m - ~ * s - ) which were automatically recorded by the machine, with the com-
sponding assimilation rates of leaf inside the sensing head being measured simultaneously. Eight arid
sandy plant species, ( i . e . Salk psmphylla C . Wang et Ch. Y . Yang, Arternisia ordosica
Krasch , Hedysanun scopariwn Fisch . et Mey . , H. laeue Maxim. , Caragana intermedia Kuang et
H. C. Fu, Sabina vulgaris Ant. , C - h k o b i Al-Iljinski , Psammochloa mongolica
Hitche) were tested by this simple method with good results. During each measurement, envimn-
mental variables such as air temperature, air humidity and C@ concentration but not including leaf
temperature changed little, and there existed a si
gnifi
cant cornlation between the measured data and
the predicted data.
Key words A-light curves, Photosynthesis, Natural light intensity, Quick measurement
@%N%$#R-%~Efi%E%+#H~%%%B$&%&%PJEEm%,SHm
gma~ax+T~la@%stJxe~;tl~~~gH. ~ h t a k . ~ u i t g # ~ l ~ ~ s ~ s l % + m , g
(light composition point, LCP) , %@!%I ,6 ( light saturation point, LSP ) , 33 A S %% f% $J H 2i
$$Sif FB$(maximum quantum yield of C Q fmtion, , @ FF @Z#* ( d d respiration
mte, ~ ) W ~ ~ % $ ~ $ ( ~ r n u m assimilation rate, A=) (Long % , 1993 ; Jiang 1997) BI
m~aa%efi~-%n(;iJEmBg@Bsleaa&+~~~s+~-~-t-+sw N.WF~XI~;J$.
k~.f)B%&WXE&Zh%~i&,%HAI%%(BE~%%:kT)$l~-%F~&%MB
$,f&G@IZ@@MElKl%$#R(~artak %, 19%; Fischer, 1997), %@ft%@JgP?ik%
B&$?@@&Yt;%i+,H%~~~%I%~~R&~~X~@&~~;Z~A~~@3X-
El h&Hfl$IRII4 El $%3%F&%e#Hjff%&X$#FB-XaltilGm%(Eang %, 1995; !JiD
&a%, 19961, iHm%k&%,62r4&gG%&&i+@B&90 BZ%%&%&#HiJ!
X%%$!n ~1-6400(%H), L C A 4 ( 8 a ) , CID-~O~PS(%H)-~~~~T%~I@~%%$#R-%~( ;~J
E~%&%~sle%%,.I&B9b%i4T#!!X,iB~2R&%&E$Jo H W , 3 E % % & %
BRHALa%@m4F $ %%,R;H.M% tLSB W , Bl%i%%%P#&S%&#R@!JX&
&%mi40 %3\,-B&H% $R@@&%+#mwBl?ik2%%&%sszR&%m&
m t f i m f i ~ - % n f i G m m @ m o S Z , % # I & E ~ ~ B I X A B @ B ~ % + # H W , E
%?-Hfi$JH@%S%&#HBdZ%%& I2 f&%,%B$TR$#%e#H-%nf iGm%
&%%,%&.I&Bfi&%+#H@dZ%B&S~k%B,&~@$nAI%%%I%B2
g o M~~&?T~9b$%%&TWX%+#R-%~EELB%&~~,%.WF%kf @#$I&
$%&t;%&TstJ%+f%;tl%@?-#~@,93RXHH.IS&fr%o
1.1 *&i*HNB%%
@H LCA4%$#H#!L!X%%(ADC, Hoddesdon, % B ) % f i E E B B & W a o i%l)lC
%IEm C 4 jF;n Hz0 a3b#fif(PG&Z~k,mWZ%%@B%&H-Y~ko €WI%%R
B$&95I~#%g.#R~YLW , % @ R % ~ & % ~ B E I P % @ % % W ~ ~ ~ % & # ~ - J & % S % $
%Kg$, %&#H#!da%%H$5% C 4 5 % ( 6 4 0 pnol*mol-, @lT;lL%#fifl#l-6
%5#$~b)&58a3\.fi;c& CQ #8?ix;H w G 6 0 2 ~ , & ~ ~ % Y % ( A D C , Hoddesdon, %
El )jE;iX Hz0 . f ) f i # o RiX%&BJ4F%%% & I # % & G ~ ~ @ % ! ~ ~ ~ X ~ # H - % V ~ E I %
&@ax,
1.2 jwi*a]-*mmemwa
~%wX~+BN%E%mH%fiI4%&@%sB~B%~%Y&X~Pti31iO B9X
%E&+%$B 2.5 T, %%%7k@i 345 mrn;+%T& $ %%JfiB$ > 2200 p n ~ l . r n - ~ .
s - , Sk.I& 2500 p n ~ l * m - ~ * s - . #!IZid@+ZZ4;%B%k&%YL~B%S lo%%&
C,%@%FI#P(Saliz p s ~ h y b C. W a g et Ch. Y . Y a n g ) . ~ ~ ( A r t e m i s i a ordosica
~ r a s c h ) , ~ ~ ( ~ e d ~ s a ~ ~ ~ p a r i u m Fisch. et Mey. ),%%(H. laeve ~auim.),+W%@,(L
( Caragana intermedia Kuang et H . C . Fn ) . @ % El ( Sabina vulgaris Ant. ) . + J L ~ $b 3 ( Cy-
nunchum komarovii Al-Iljinski ) , %I Cq @ % @ @F ( P s m h mongolica Hitchc . ) , #!I X & f?$
$RB%: S@WIUViti!$%i&HH EI % ,%3WS%ARA%%MRE9%q9-%34M%
,M2E$o %&%:%@WSa%@%(B&Sl4-YE,TB%WS)%%!8%R&B,%
%sk%,aB$,SN%B%Hka@e%%%ZGa4q9Bkr9,%eB$,%Ga3RA
BMkr;iJ%3Wg ,%19@1B-&MX3Ba#Gr9,%e@ @ltt;%#@,X@%3fiEM;I;
H@BJ% 2200, 2000, 1700, 1500, 1250, 1000, 750, 500, 350, 200, 100, 50, 0 p n ~ l - r n - ~
.s-H%3LI%QWB(GHM%QMBSH%kB/7.;#WT%e#H%@iZ3TX) ,#%
%%lEH~%e@o H%GSB%aHEE%%,RS%3WS%U%q%@1BM%%%
E ( < 100 p n ~ l . r n - ~ . s - ) , %HB;ffiZMB3%*&12WS,X$%3J4bXRM%3BUE 0
~ ~ ~ m ~ 2 ~ s ~ ~ ~ ~ % @ ~ o $E@+%?8ikX ,S%%%? 3 ?%Y%7WZo ---&--%%e@H-
%nAGW&gJ& 15min Vit%Fifi,4b?#iBBo %H@#B%6 3 - 5 &BBo
1.3 #&e&E
&%k%WB$T ,XK&aHkZSBJHGH@%e#HM%k,@@@%ZG,
~ $ ~ ~ W Z M Z M W @ L P X G A ~ I ~ H ~ ~ , % + @ @ R G ~ W B @ ~ R % B B $ , ~ T L ~ E , @
El CQ %E,WE~E,@#GMsr;%&#bft3nX,%B$,k$ c 4 %E,k$BB,k$B
E, k$E%, ?&#%a3 LCA4 Hg@&SiJ%Ait$f#L,H Excell 97 @@%%J%e#R-%
@lEWi%, Fl%EBB#%3Ij. ,K!%%kXU%Fr LCP, LSP, @m,,p,%, A,%~
. t s ~ " " ~ " " " ~
-I#, m sm lom Irm lam zm
35
"
21
: 10
5 .
0
4
@UY%B$$Bi%o $%%W!Z@W%i!k$%&T 2 - 6 p o l ~@.rn-~.s- &I 6l ,y@ 3.5
F
-
. .. .-.
- i--- . " t .
-
-
* 1 .Q p o l . c @ rn-2*s- o fik%.f).&%Jb9@iVERi!k$ < 2 p o l ~ @ - r n - ~ * s - ~ $A%&%
:~Z%@%IG$@~~~#%-~;;~BBA 71gk .f).&%&mXSZ@iWa%?fi%#To Z?i#S3!6&!I
~"@iv%~RB%EBBa%ET?%B&!I%%&B&&&MG#o E~&%%%ETwi
I .am I#, " " " " Sm loo0 I4m lam " - pm
XR$S& ( u mol . m-2 .s -)
2 Ei3LZH%dJWZZEkR%+tt5 6@.W.&@CtYJYt&RM-%@IJGi%%
A . @#P; B. +%; C. B~Ldb3; D. miFg; E. a#; F. ?kBtd,
4) 6m@Hfk&E&&#%e#H-%flm&m%5Ho ~ ~ \ T l 6 J % ~ ~ L , + ~ L ~ ~ b
F ,%173,@@P.%%%%S$41!& C, &#J,WG@&&%+EE,&(~OO - 200 w l * m - 2 . s - 1 )
$U@$U,&(+%Il&~%@$U,&&%B> , @ @ % g b j E B ( > 2 p o l c Q . m - s- ) ;m@@
Jil!%3!i!LbWB!N G %$E,$E%@f&%T( < 5 0 p o l ~ O z r n - ~ S - ) ~ ~ N ~ S ~ ~ J ~ ~ # M E
&,fiR%t&$UkW!lBB( > 1500 p~l.rn-~.s-~),!H@@%%$B@,@~JZ@a - 1 . 2
pnol C O ~ - ~ - ~ . S - ~ & I ? ~ @MH&$Z%T&&&3!i!R@,HS<@B&@V%g$(9&&
2- 6 pmol C Q . ~ - ~ . S - ~ I ~ ) ~ iF&WF%X%H&@WfE$Z&TBSEflBAjMIBB7k%
$JHB$(%%%%, 1992; g%%, 1 9 8 ) , f H X $ ~ % e % , & @ % % @ , @ M ~ ~ % 3 ! i ! &
%*&%e#H-%nfiEfi%4Fe@i9fiBBi$o

~ifi48tb:,g@%,%%,1996. @@&%+)tit, 20:225 - 234
H&%,AF%, 1996. @@ifi4&W)tit, 20:120- 131
gBsa,Hjw, 1997. NWB~S~, 42:434-438
BWV,&%%,88W,9J&?X9 W8GB%,96P!,BR%, 1994. @%l&%tfi,18:86-94
Bart& M, Nijs 1, Impens I , 1996. P h y n h i o a , 32 : 549 - 562
Fischer E S, 1997. h ~ i m , 33: 385 - 390
Liang Neishen , Nagayama M , Nakata M, k y a m a K, 1995. Phavynhicn, 31 : 257 - 26
Long S P, Baker N R , Rainses C A, 1993. Vegetatio, 1041105: 33 - 45