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作 者 ：邵寒娟 胡涌刚 丁亮 沈明山 陈睦传 张向红

期 刊 ：植物学通报 2001年 18卷 01期 页码：113-117

关键词：低能离子注入；毛细管区带电泳法；甜菊；甜菊苷；

摘 要 ：本文介绍了一种用毛细管区带电泳法筛选甜菊糖苷突变体的有效方法。根据实验结果，优化的电泳条件为：60 mmol/L Tris硼酸缓冲液(pH 8.0)，柱温30℃，工作电压25 kV。优化条件下，甜菊苷(Stevioside)迁移时间的R.S.D为0.45%(15次)，且在7.45×10-5～1.74×10-2 mol/L的浓度范围内存在良好的线性关系(r=0.9994)，甜菊主要糖苷在5 min内均可实现分离。在优化条件下，本实验研究了低能离子注入后甜菊主要糖苷含量变化，结果令人满意。

Abstract:This paper introduced an effect capillary zone electrophoresis method for screening of stevia mutation. According to the experimental results, the optimum separation conditions were selected as: 60 mmol/L Tristetraborate buffer of pH 8.0, 30 ℃ and 25 KV. Under optimum conditions, the R.S.D. of stevioside migration time for 15 runs was 0.45%. The detector response for stevioside was linear over the range of 7.45×10-5～1.74×10-2 mol/L (r=0.9994). Stevia main sweeteners cound be separated effectively in less than five minutes. This method was applied for determining the variation of stevia main sweeteners after low_energy ions implantation.

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